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Oct4 antibody detects Oct4 protein at nucleus by immunofluorescent analysis. Sample: NT2D1 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Oct4 stained by Oct4 antibody (GTX100622) diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar= 10 μm.
Oct4 antibody detects Oct4 protein at nucleus by immunofluorescent analysis. Sample: NT2D1 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Oct4 stained by Oct4 antibody (GTX100622) diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar= 10 μm.
Oct4 antibody detects Oct4 protein at nucleus by immunofluorescent analysis. Sample: NT2D1 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Oct4 stained by Oct4 antibody (GTX100622) diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar= 10 μm.

Oct4 antibody

GTX100622
GeneTex
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetPOU5F1
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Overview

  • Supplier
    GeneTex
  • Product Name
    Oct4 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:1000-1:20000. ICC/IF: 1:100-1:1000. FACS: 1:50-1:200. IP: 1:500-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Flow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.32 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID5460
  • Target name
    POU5F1
  • Target description
    POU class 5 homeobox 1
  • Target synonyms
    OCT3, OCT4, OCT4Borf1, OTF-3, OTF3, OTF4, Oct-3, Oct-4, Oct3/4, POU domain, class 5, transcription factor 1, POU domain transcription factor OCT4, POU-type homeodomain-containing DNA-binding protein, octamer-binding protein 3, octamer-binding protein 4, octamer-binding transcription factor 3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ01860
  • Protein Name
    POU domain, class 5, transcription factor 1
  • Scientific Description
    This gene encodes a transcription factor containing a POU homeodomain. This transcription factor plays a role in embryonic development, especially during early embryogenesis, and it is necessary for embryonic stem cell pluripotency. A translocation of this gene with the Ewings sarcoma gene, t(6;22)(p21;q12), has been linked to tumor formation. Alternative splicing, as well as usage of alternative translation initiation codons, results in multiple isoforms, one of which initiates at a non-AUG (CUG) start codon. Related pseudogenes have been identified on chromosomes 1, 3, 8, 10, and 12. [provided by RefSeq]
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Blanas A, Zaal A, van der Haar Àvila I, et al. FUT9-Driven Programming of Colon Cancer Cells towards a Stem Cell-Like State. Cancers (Basel). 2020,12(9). doi: 10.3390/cancers12092580
    Read this paper
  • O'Day E, Le MTN, Imai S, et al. An RNA-binding Protein, Lin28, Recognizes and Remodels G-quartets in the MicroRNAs (miRNAs) and mRNAs It Regulates. J Biol Chem. 2015,290(29):17909-17922. doi: 10.1074/jbc.M115.665521
    Read this paper
  • Chen PM, Cheng YW, Wang YC, et al. Up-regulation of FOXM1 by E6 oncoprotein through the MZF1/NKX2-1 axis is required for human papillomavirus-associated tumorigenesis. Neoplasia. 2014,16(11):961-71. doi: 10.1016/j.neo.2014.09.010
    Read this paper

Datasheet

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