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Non-transfected (–) and transfected (+) NT2D1 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Oct4 antibody (GTX101497) diluted at 1:200000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Non-transfected (–) and transfected (+) NT2D1 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Oct4 antibody (GTX101497) diluted at 1:200000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Non-transfected (–) and transfected (+) NT2D1 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Oct4 antibody (GTX101497) diluted at 1:200000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Oct4 antibody

GTX101497
GeneTex
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetPOU5F1
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Overview

  • Supplier
    GeneTex
  • Product Name
    Oct4 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:5000-1:100000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. FACS: 1:50-1:200. IP: 1:500-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Flow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID5460
  • Target name
    POU5F1
  • Target description
    POU class 5 homeobox 1
  • Target synonyms
    OCT3, OCT4, OCT4Borf1, OTF-3, OTF3, OTF4, Oct-3, Oct-4, Oct3/4, POU domain, class 5, transcription factor 1, POU domain transcription factor OCT4, POU-type homeodomain-containing DNA-binding protein, octamer-binding protein 3, octamer-binding protein 4, octamer-binding transcription factor 3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ01860
  • Protein Name
    POU domain, class 5, transcription factor 1
  • Scientific Description
    This gene encodes a transcription factor containing a POU homeodomain. This transcription factor plays a role in embryonic development, especially during early embryogenesis, and it is necessary for embryonic stem cell pluripotency. A translocation of this gene with the Ewings sarcoma gene, t(6;22)(p21;q12), has been linked to tumor formation. Alternative splicing, as well as usage of alternative translation initiation codons, results in multiple isoforms, one of which initiates at a non-AUG (CUG) start codon. Related pseudogenes have been identified on chromosomes 1, 3, 8, 10, and 12. [provided by RefSeq]
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Tsai YF, Chan LP, Chen YK, et al. RAD51 is a poor prognostic marker and a potential therapeutic target for oral squamous cell carcinoma. Cancer Cell Int. 2023,23(1):231. doi: 10.1186/s12935-023-03071-w
    Read this paper
  • Wang CH, Huang YF, Shyu WC, et al. Cbx7 promotes the generation of induced pluripotent stem cells. Regen Ther. 2023,24:443-450. doi: 10.1016/j.reth.2023.09.008
    Read this paper
  • Cardile A, Passarini C, Zanrè V, et al. Hyperforin Enhances Heme Oxygenase-1 Expression Triggering Lipid Peroxidation in BRAF-Mutated Melanoma Cells and Hampers the Expression of Pro-Metastatic Markers. Antioxidants (Basel). 2023,12(7). doi: 10.3390/antiox12071369
    Read this paper
  • Ye P, Chi X, Yan X, et al. Alanine-Glyoxylate Aminotransferase Sustains Cancer Stemness Properties through the Upregulation of SOX2 and OCT4 in Hepatocellular Carcinoma Cells. Biomolecules. 2022,12(5). doi: 10.3390/biom12050668
    Read this paper
  • Zhang W, Li Y, Wang L, et al. Generation of a DKK2 homozygous knockout human embryonic stem cell line using the CRISPR/Cas9 system. Stem Cell Res. 2021,57:102611. doi: 10.1016/j.scr.2021.102611
    Read this paper
  • Mohankumar K, Shrestha R, Safe S. Nuclear receptor 4A1 (NR4A1) antagonists target paraspeckle component 1 (PSPC1) in cancer cells. Mol Carcinog. 2022,61(1):73-84. doi: 10.1002/mc.23362
    Read this paper
  • Salti A, Etemad S, Cubero MS, et al. High Glycolytic Activity Enhances Stem Cell Reprogramming of Fahd1-KO Mouse Embryonic Fibroblasts. Cells. 2021,10(8). doi: 10.3390/cells10082040
    Read this paper
  • He Q, Song J, Cui C, et al. Mesenchymal stem cell-derived exosomal miR-146a reverses diabetic β-cell dedifferentiation. Stem Cell Res Ther. 2021,12(1):449. doi: 10.1186/s13287-021-02371-0
    Read this paper
  • Wang YY, Chen HD, Lo S, et al. Visfatin Enhances Breast Cancer Progression through CXCL1 Induction in Tumor-Associated Macrophages. Cancers (Basel). 2020,12(12). doi: 10.3390/cancers12123526
    Read this paper
  • Fang W, Ni M, Zhang M, et al. Prognostic value of OCT4 in colorectal cancer: analysis using immunohistochemistry and bioinformatics validation. Biomark Med. 2020,14(15):1473-1484. doi: 10.2217/bmm-2020-0069
    Read this paper

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Figure 1. Western blot analysis of POU5F1 using anti-POU5F1 antibody (A00174-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: rat brain tissue lysates, Lane 3: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-POU5F1 antigen affinity purified polyclonal antibody (Catalog # A00174-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for POU5F1 at approximately 45 kDa. The expected band size for POU5F1 is at 39 kDa.
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