More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/PathPlus(tm) KRT8 / CK8 / Cytokeratin 8 Antibody

PathPlus(tm) KRT8 / CK8 / Cytokeratin 8 Antibody

LS-B16143
LifeSpan BioSciences
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetKRT8
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    PathPlus(tm) KRT8 / CK8 / Cytokeratin 8 Antibody
  • Delivery Days Customer
    14
  • Application Supplier Note
    Use Heat-induced epitope retrieval (HIER) TRIS-EDTA of pH 8.0 Recommended IHC controls tissues are Cholangiocarcinoma, Liver, gastric adenocarcinoma, Kindey. Resulting localization is Cytoplasmic, Membranous. IHC (1:200 - 1:400), IHC-P (1:50), WB (1:200 - 1:400) Use Heat-induced epitope retrieval (HIER) TRIS-EDTA of pH 8.0 Recommended IHC controls tissues are Cholangiocarcinoma, Liver, gastric adenocarcinoma, Kindey. Resulting localization is Cytoplasmic, Membranous
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID3856
  • Target name
    KRT8
  • Target description
    keratin 8
  • Target synonyms
    CARD2, CK-8, CK8, CYK8, K2C8, K8, KO, keratin, type II cytoskeletal 8, cytokeratin-8, keratin 8, type II, type-II keratin Kb8
  • Host
    Mouse
  • Isotype
    IgG
  • Reactivity
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

FAQ

Go to our frequently asked questions

Related products

Product group Antibodies
Absolute Antibody
Anti-Human Cytokeratin 8 [1E8-C6-B4]Ab02687-1.1
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot
ReactivityHuman
TargetKRT8
  • SizePrice
Product group Antibodies
RayBiotech
Anti-KRT8 Antibody130-10237
ApplicationsWestern Blot, ELISA
  • SizePrice
Product group Antibodies
Biosynth
Cytokeratin 8 antibody10R-2766
  • SizePrice
Figure 1. Western blot analysis of Cytokeratin 8 using anti-Cytokeratin 8 antibody (A01421). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: mouse ovary tissue lysates, Lane 3: mouse liver tissue lysates, Lane 4: MCF-7 whole cell lysates, Lane 5: A549 whole cell lysates, Lane 6: HELA whole Cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cytokeratin 8 antigen affinity purified polyclonal antibody (Catalog # A01421) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cytokeratin 8 at approximately 54KD, 56KD. The expected band size for Cytokeratin 8 is at 54KD.
Product group Antibodies
Boster Bio
Anti-Cytokeratin 8/KRT8 Antibody Picoband(r)A01421-CARRIER-FREE
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetKRT8
  • SizePrice
Cytokeratin 8 antibody detects Cytokeratin 8 protein at cytoplasm by immunofluorescent analysis. Sample: MDCK cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Cytokeratin 8 stained by Cytokeratin 8 antibody (GTX109489) diluted at 1:500.
Product group Antibodies
References
GeneTex
Cytokeratin 8 antibody [N1N3]GTX109489
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityCanine, Human, Mouse, Rat, Zebra Fish
TargetKRT8
  • SizePrice
Product group Antibodies
Biomatik
Krt8 Polyclonal AntibodyCAC07095
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
TargetKRT8
  • SizePrice
Formalin-fixed and rat colon tissue labeled with Anti-CK8 Polyclonal Antibody, Unconjugated (bs-1106R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Product group Antibodies
References
Bioss
Cytokeratin 8 Polyclonal AntibodyBS-1106R
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
TargetKRT8
  • SizePrice
Immunohistochemical staining of formalin fixed and paraffin embedded human breast cancer tissue sections using Anti-CK8 Rabbit Monoclonal Antibody (Clone RM266) at a 1:2000 dilution.
Product group Antibodies
RevMAb Biosciences
anti-Cytokeratin-8 (human), Rabbit Monoclonal (RM266)REV-31-1148-00
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman
TargetKRT8
  • SizePrice
Anti-Keratin 8 Antibody
Product group Antibodies
Antibodies.com
Anti-Keratin 8 AntibodyA95180
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
  • SizePrice
Product group Antibodies
Assay Genie
RORC AntibodyCAB10240
ReactivityHuman
TargetKRT8
  • SizePrice