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IHC-P analysis of human heart tissue using GTX31308 PD-L1 antibody. Working concentration : 20 microg/ml
IHC-P analysis of human heart tissue using GTX31308 PD-L1 antibody. Working concentration : 20 microg/ml
IHC-P analysis of human heart tissue using GTX31308 PD-L1 antibody. Working concentration : 20 microg/ml

PD-L1 antibody

GTX31308
GeneTex
ApplicationsFlow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetCD274
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Overview

  • Supplier
    GeneTex
  • Product Name
    PD-L1 antibody - KO/KD, Overexpression Validated
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 0.5 - 1 microg/mL. IHC-P: 2.5 microg/mL. FACS: 0.5 microg/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Flow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID29126
  • Target name
    CD274
  • Target description
    CD274 molecule
  • Target synonyms
    B7 homolog 1; B7-H; B7H1; CD274 antigen; hPD-L1; PDCD1 ligand 1; PDCD1L1; PDCD1LG1; PDL1; PD-L1; programmed cell death 1 ligand 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9NZQ7
  • Protein Name
    Programmed cell death 1 ligand 1
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • PD-L1-mediated immune evasion in triple-negative breast cancer is linked to the loss of ZNF652.
    Read more
  • WSX1 act as a tumor suppressor in hepatocellular carcinoma by downregulating neoplastic PD-L1 expression. Wu M et al., 2021 Jun 9, Nat Commun
    Read more
  • Inhibition of TRPV1 by SHP-1 in nociceptive primary sensory neurons is critical in PD-L1 analgesia. Liu BL et al., 2020 Oct 15, JCI Insight
    Read more
  • The extracts of Astragalus membranaceus overcome tumor immune tolerance by inhibition of tumor programmed cell death protein ligand-1 expression. Chang HL et al., 2020, Int J Med Sci
    Read more
  • NPM1 upregulates the transcription of PD-L1 and suppresses T cell activity in triple-negative breast cancer. Qin G et al., 2020 Apr 3, Nat Commun
    Read more
  • The prediction of survival of patients with gastric cancer with PD-L1 expression using contrast-enhanced ultrasonography. Wang LA et al., 2016 Jun, Tumour Biol
    Read more

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Figure 1. Western blot analysis of PD-L1/B7-H1 using anti-PD-L1/B7-H1 antibody (A00109). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: HELA whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PD-L1/B7-H1 antigen affinity purified polyclonal antibody (Catalog # A00109) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PD-L1/B7-H1 at approximately 62KD. The expected band size for PD-L1/B7-H1 is at 33KD.
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