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WB analysis of membrane enriched extracts (30 microg lysate) of A549 (Lane 1), A-431 (Lane 2), HeLa (Lane 3), MCF7 (Lane 4), K-562 (Lane 5), Caco-2 (Lane 6), Hep G2 (Lane 7) and COLO 205 (Lane 8) using GTX11432 PDIA6 antibody. Dilution : 1:250
WB analysis of membrane enriched extracts (30 microg lysate) of A549 (Lane 1), A-431 (Lane 2), HeLa (Lane 3), MCF7 (Lane 4), K-562 (Lane 5), Caco-2 (Lane 6), Hep G2 (Lane 7) and COLO 205 (Lane 8) using GTX11432 PDIA6 antibody. Dilution : 1:250
WB analysis of membrane enriched extracts (30 microg lysate) of A549 (Lane 1), A-431 (Lane 2), HeLa (Lane 3), MCF7 (Lane 4), K-562 (Lane 5), Caco-2 (Lane 6), Hep G2 (Lane 7) and COLO 205 (Lane 8) using GTX11432 PDIA6 antibody. Dilution : 1:250

PDIA6 antibody

GTX11432
GeneTex
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
DownloadDatasheet
Product group Antibodies
TargetPDIA6
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Overview

  • Supplier
    GeneTex
  • Product Name
    PDIA6 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:250. ICC/IF: 1:250. FACS: 1:20. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID10130
  • Target name
    PDIA6
  • Target description
    protein disulfide isomerase family A member 6
  • Target synonyms
    endoplasmic reticulum protein 5; epididymis secretory sperm binding protein; ER protein 5; ERP5; P5; protein disulfide isomerase P5; protein disulfide isomerase-associated 6; protein disulfide isomerase-related protein; protein disulfide-isomerase A6; thioredoxin domain containing 7 (protein disulfide isomerase); thioredoxin domain-containing protein 7; TXNDC7
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ15084
  • Protein Name
    Protein disulfide-isomerase A6
  • Scientific Description
    This gene encodes a member of the disulfide isomerase (PDI) family of endoplasmic reticulum (ER) proteins that catalyze protein folding and thiol-disulfide interchange reactions. The encoded protein has an N-terminal ER-signal sequence, two catalytically active thioredoxin (TRX) domains, a TRX-like domain, and a C-terminal ER-retention sequence. This protein inhibits the aggregation of misfolded proteins and exhibits both isomerase and chaperone activity. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Dec 2016]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PDIA6 using anti-PDIA6 antibody (A03813-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human HT1080 whole cell lysates, Lane 4: human Raji whole cell lysates, Lane 5: human Hela whole cell lysates, Lane 6: human HEK293 whole cell lysates, Lane 7: human THP-1 whole cell lysates, Lane 8: human SH-SY5Y whole cell lysates, Lane 9: rat kidney tissue lysates, Lane 10: rat heart tissue lysates, Lane 11: rat liver tissue lysates, Lane 12: rat lung tissue lysates, Lane 13: mouse kidney tissue lysates, Lane 14: mouse heart tissue lysates, Lane 15: mouse liver tissue lysates, Lane 16: mouse lung tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PDIA6 antigen affinity purified polyclonal antibody (Catalog # A03813-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PDIA6 at approximately 50KD. The expected band size for PDIA6 is at 50KD.
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