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Immunohistochemistry of paraffin-embedded Human lymphoma tissue using PIK3R4 Polyclonal Antibody at dilution 1:85
Immunohistochemistry of paraffin-embedded Human lymphoma tissue using PIK3R4 Polyclonal Antibody at dilution 1:85
Immunohistochemistry of paraffin-embedded Human lymphoma tissue using PIK3R4 Polyclonal Antibody at dilution 1:85

PIK3R4 Polyclonal Antibody

E-AB-16201
Elabscience
Product group Antibodies
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Overview

  • Supplier
    Elabscience
  • Product Name
    PIK3R4 Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications Supplier
    ELISA IHC
  • Certification
    Research Use Only
  • Concentration
    0.4mg/ml
  • Scientific Description
    Phosphoinositide 3-kinase regulatory subunit 4, also known asPI3-kinase regulatory subunit 4orPI3-kinase p150 subunitorphosphoinositide 3-kinase adaptor protein, orVPS15is an enzyme that in humans is encoded by thePIK3R4gene. The PI3-kinases regulate cellular signaling networks that are involved in processes linked to the survival, growth, proliferation, metabolism and specialized differentiated functions of cells. The subversion of this network is common in cancer and has also been linked to disorders of inflammation.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of VPS15/PIK3R4 using anti-VPS15/PIK3R4 antibody (A06618-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VPS15/PIK3R4 antigen affinity purified polyclonal antibody (Catalog # A06618-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for VPS15/PIK3R4 at approximately 150 kDa. The expected band size for VPS15/PIK3R4 is at 153 kDa.
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