ICC/IF analysis of COS7 cells transiently transfected with PLK1 plasmid using GTX83866 PLK1 antibody [8C12]. Dilution : 1:100
![IP analysis of DDDDK tagged PLK1 overexpressed HEK293T lysate using GTX83866 PLK1 antibody [8C12].After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDDDK polyclonal antibody. IP reaction : 2microg antibody / 500microl cell lysate](https://www.genetex.com/upload/website/prouct_img/normal/GTX83866/GTX83866_3387_IP_w_23061420_751.webp "IP analysis of DDDDK tagged PLK1 overexpressed HEK293T lysate using GTX83866 PLK1 antibody [8C12].After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDDDK polyclonal antibody. IP reaction : 2microg antibody / 500microl cell lysate")
![WB analysis of HEK293T cells transfected with PLK1 plasmid (Right) or empty vector (Left) for 48 hrs using GTX83866 PLK1 antibody [8C12]. Loading : 5 ug per lane Dilution : 1:500](https://www.genetex.com/upload/website/prouct_img/normal/GTX83866/GTX83866_4408_WB_w_23061420_131.webp "WB analysis of HEK293T cells transfected with PLK1 plasmid (Right) or empty vector (Left) for 48 hrs using GTX83866 PLK1 antibody [8C12]. Loading : 5 ug per lane Dilution : 1:500")
![WB analysis of various cell lines using GTX83866 PLK1 antibody [8C12]. Loading : 35 ug per lane Dilution : 1:500](https://www.genetex.com/upload/website/prouct_img/normal/GTX83866/GTX83866_4436_WB_w_23061420_734.webp "WB analysis of various cell lines using GTX83866 PLK1 antibody [8C12]. Loading : 35 ug per lane Dilution : 1:500")
![FACS analysis of HEK293T cells transfected with either PLK1 plasmid(Red) or empty vector control plasmid(Blue) using GTX83866 PLK1 antibody [8C12]. Dilution : 1:100](https://www.genetex.com/upload/website/prouct_img/normal/GTX83866/GTX83866_535_FACS_w_23061420_482.webp "FACS analysis of HEK293T cells transfected with either PLK1 plasmid(Red) or empty vector control plasmid(Blue) using GTX83866 PLK1 antibody [8C12]. Dilution : 1:100")
ICC/IF analysis of COS7 cells transiently transfected with PLK1 plasmid using GTX83866 PLK1 antibody [8C12]. Dilution : 1:100
PLK1 antibody [8C12]
GTX83866
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityCanine, Human, Monkey
TargetPLK1
Overview
- SupplierGeneTex
- Product NamePLK1 antibody [8C12] - Overexpression Validated
- Delivery Days Customer9
- Application Supplier NoteWB: 1:2000. ICC/IF: 1:100. FACS: 1:100. IP: 2ug/500ul. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID8C12
- Concentration0.96 mg/ml
- ConjugateUnconjugated
- Gene ID5347
- Target namePLK1
- Target descriptionpolo like kinase 1
- Target synonymscell cycle regulated protein kinase; PLK; PLK-1; polo (Drosophia)-like kinase; serine/threonine-protein kinase 13; serine/threonine-protein kinase PLK1; STPK13
- HostMouse
- IsotypeIgG1
- Protein IDP53350
- Protein NameSerine/threonine-protein kinase PLK1
- Scientific DescriptionSerine/threonine-protein kinase that performs several important functions throughout M phase of the cell cycle, including the regulation of centrosome maturation and spindle assembly, the removal of cohesins from chromosome arms, the inactivation of APC/C inhibitors, and the regulation of mitotic exit and cytokinesis. Required for recovery after DNA damage checkpoint and entry into mitosis. Required for kinetochore localization of BUB1B. Phosphorylates SGOL1. Required for spindle pole localization of isoform 3 of SGOL1 and plays a role in regulating its centriole cohesion function. Phosphorylates BORA, and thereby promotes the degradation of BORA. Contributes to the regulation of AURKA function. Regulates TP53 stability through phosphorylation of TOPORS.
- ReactivityCanine, Human, Monkey
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203