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Product group Antibodies
OriGene
EGFR pTyr869 Mouse Monoclonal Antibody [Clone ID: 12A3]AM00031FC-N
ApplicationsImmunoFluorescence
ReactivityHuman, Mouse
TargetEGFR
  • SizePrice
Phosphospecificity: Whole cell extracts of control (co), EGF stimulated (EGF) or pervanadate treated (VH) HepG2 and A549 tumor cells were applied to SDS-PAGE (ca 20.000 cells per lane) and transferred to a PVDF membrane. The immunoblot was probed with mab EGFR-12A3 (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec).
Product group Antibodies
OriGene
EGFR pTyr869 (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 12A3]AM00031PU-N
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse
TargetEGFR
  • SizePrice
Product group Antibodies
OriGene
EGFR (960-980) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 16F8]AM00033BT-N
ApplicationsWestern Blot
ReactivityCanine, Human, Mouse, Rat
TargetEGFR
  • SizePrice
Antibody sensitivity Whole cell lysates of vanadate - treated HepG2 containing defined cell numbers per lane were applied to SDS-PAGE and transferred to PVDF membranes. Immunoblots were probed with mab EGFR-16F8 (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). lane 1: 160.000 cells, lane 2: 80.000 cells lane 3: 40.000 cells, lane 4: 20.000 cells, lane 5: 10.000 cells, lane 6: 5.000 cells, lane 7: 2.500 cells, lane 8: 1.000 cells
Product group Antibodies
OriGene
EGFR (960-980) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 16F8]AM00033PU-N
ApplicationsWestern Blot
ReactivityCanine, Human, Mouse, Rat
TargetEGFR
  • SizePrice
Product group Antibodies
OriGene
EGFR pTyr1069 (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 11C2]AM00034BT-N
ApplicationsImmunoPrecipitation, Western Blot, ELISA
ReactivityHuman, Mouse
TargetEGFR
  • SizePrice
EGFR Activation Serum starved MDA-MB 468 cells were incubated with 10 ng/ml EGF for the indicated times. Whole cell lysates were prepared with lysis buffer V19 and separated by SDS-PAGE (ca 20.000 cells/lane). The immunoblot was probed with mab EGFR-11C2 (0.5 ug/ ml) for 1 h at RT and developed by ECL (exp. time: 30 sec). Lane 1: Control; Lane 2: 5 min EGF; Lane 3: 15 min EGF; Lane 4: 30 min EGF; Lane 5: 1h EGF; Lane 6; 2h EGF; Lane 7: 4h EGF; Lane 8: 8h EGF.
Product group Antibodies
OriGene
EGFR pTyr1069 (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 11C2]AM00034PU-N
ApplicationsImmunoPrecipitation, Western Blot, ELISA
ReactivityHuman, Mouse
TargetEGFR
  • SizePrice
Product group Antibodies
OriGene
EGFR pSer1071 (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 1H9]AM00036BT-N
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA
ReactivityCanine, Human, Mouse
TargetEGFR
  • SizePrice
EGFR Transactivation: Serum starved HepG2 cells were treated for 15min as indicated. Whole cell lysates were separated by SDS-PAGE (ca 20.000 cells/lane). The immunoblot was probed with mab EGFR-1H9 (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). Lane 1: Control Lane 2: PMA Lane 3: Forskolin Lane 4: LPA Lane 5: Sorbit Lane 6; Anisomycin Lane 7: Ionomycin Lane 8: Taxol
Product group Antibodies
OriGene
EGFR pSer1047 (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 1H9]AM00036PU-N
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA
ReactivityCanine, Human, Mouse
TargetEGFR
  • SizePrice
Product group Antibodies
OriGene
EGFR pTyr1092 (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 15A2]AM00037BT-N
ApplicationsImmunoPrecipitation, Western Blot, ELISA
ReactivityCanine, Human, Mouse, Rat
TargetEGFR
  • SizePrice
Phosphospecificity: Whole cell extracts of control (co), EGF stimulated (EGF) or pervanadate treated (VH) SKOV3 tumor cells were applied to SDS-PAGE (ca 20.000 cells per lane) and transferred to a PVDF membrane. The immunoblot was probed with mab EGFR-15A2 (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec).
Product group Antibodies
OriGene
EGFR pTyr1092 (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 15A2]AM00037PU-N
ApplicationsImmunoPrecipitation, Western Blot, ELISA
ReactivityCanine, Human, Mouse, Rat
TargetEGFR
  • SizePrice
Product group Antibodies
OriGene
EGFR pTyr1110 Mouse Monoclonal Antibody [Clone ID: 8B8]AM00039BT-N
ApplicationsWestern Blot, ELISA
ReactivityHuman
TargetEGFR
  • SizePrice
Phosphospecificity Whole cell extracts of control (co), EGF stimulated (EGF) or pervanadate treated (VH) MDA-MB 468 tumor cells were applied to SDS-PAGE (ca 20.000 cells per lane) and transferred to a PVDF membrane. The immunoblot was probed with mab EGFR-8B8 (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec).
Product group Antibodies
OriGene
EGFR pTyr1110 Mouse Monoclonal Antibody [Clone ID: 8B8]AM00039PU-N
ApplicationsWestern Blot, ELISA
ReactivityHuman
TargetEGFR
  • SizePrice
34567

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