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Product group Antibodies
OriGene
EGFR (1140-1160) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 10F4]AM00040BT-N
ApplicationsWestern Blot
ReactivityHuman, Mouse
TargetEGFR
  • SizePrice
Antibody sensitivity Whole cell lysates of vanadate-treated HepG2 containing defined cell numbers per lane were applied to SDS-PAGE and transferred to PVDF membranes. Immunoblots were probed with mab AM00040PU-N (0.5 ug/ ml) for 1 h at RT and developed by ECL (exp. time: 30 sec). lane 1: 160.000 cells, lane 2: 80.000 cells lane 3: 40.000 cells, lane 4: 20.000 cells, lane 5: 10.000 cells, lane 6: 5.000 cells, lane 7: 2.500 cells, lane 8: 1.000 cells
Product group Antibodies
OriGene
EGFR (1140-1160) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 10F4]AM00040PU-N
ApplicationsWestern Blot
ReactivityHuman, Mouse
TargetEGFR
  • SizePrice
Detection of endogenous EGFR Whole cell lysates of EGF-stimulated tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with AM00041PU-N (0.5 ug/ ml) for 1 h at RT and developed by ECL (exp. time: 30 sec). lane 1: A431; lane 2: A549; lane 3: SKOV3; lane 4: OVCAR5; lane 5: HaCaT; lane 6: PC3; lane 7: HeLa; lane 8: HepG2
Product group Antibodies
OriGene
EGFR pTyr1172 (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 10G12]AM00041PU-N
ApplicationsWestern Blot
ReactivityHuman
TargetEGFR
  • SizePrice
Product group Antibodies
OriGene
EGFR pTyr1173 (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 9H2]AM00042BT-N
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityCanine, Human, Mouse
TargetEGFR
  • SizePrice
Product group Antibodies
OriGene
EGFR pTyr1173 Mouse Monoclonal Antibody [Clone ID: 9H2]AM00042FC-N
ApplicationsImmunoFluorescence
ReactivityCanine, Human, Mouse
TargetEGFR
  • SizePrice
Phosphospecificity: Whole cell extracts of control (co), EGF stimulated (EGF) or pervanadate treated (VH) A549 tumor cells were applied to SDS-PAGE (ca 20.000 cells per lane) and transferred to a PVDF membrane. The immunoblot was probed with mab EGFR-9H2 (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec).
Product group Antibodies
OriGene
EGFR pTyr1173 (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 9H2]AM00042PU-N
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityCanine, Human, Mouse
TargetEGFR
  • SizePrice
Product group Antibodies
OriGene
EGFR Non pTyr1197 (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 20G3]AM00044BT-N
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA
ReactivityHuman, Mouse
TargetEGFR
  • SizePrice
Phosphospecificity Whole cell extracts of control (co) and EGF stimulated (EGF) A431 tumor cells were applied to SDS-PAGE (ca. 20.000 cells per lane) and transferred to a PVDF membrane. The immunoblot was probed with mab EGFR-10G12 (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec).
Product group Antibodies
OriGene
EGFR Non pTyr1197 (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 20G3]AM00044PU-N
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA
ReactivityHuman, Mouse
TargetEGFR
  • SizePrice
Product group Antibodies
OriGene
EGFR (N-term) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 14C8]AM00045BT-N
ApplicationsWestern Blot
ReactivityCanine, Human, Mouse
TargetEGFR
  • SizePrice
Detection of endogenous EGFR: Whole cell lysates of EGF stimulated serum starved tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with mab EGFR-14C8 (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). lane 1: A431; lane 2: A549; lane 3: SKOV3; lane 4: OVCAR5; lane 5: HaCaT; lane 6: PC3; lane 7: HeLa; lane 8: HepG2
Product group Antibodies
OriGene
EGFR (N-term) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 14C8]AM00045PU-N
ApplicationsWestern Blot
ReactivityCanine, Human, Mouse
TargetEGFR
  • SizePrice
Product group Antibodies
OriGene
EGFR (Extracell. Dom.) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 20E12]AM00046BT-N
ApplicationsWestern Blot
ReactivityHuman
TargetEGFR
  • SizePrice
Antibody sensitivity Whole cell lysates of A431cells containing defined cell numbers per lane were applied to SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with mab EGFR-20E12 (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). lane 1: 160.000 cells, lane 2: 80.000 cells lane 3: 40.000 cells, lane 4: 20.000 cells, lane 5: 10.000 cells, lane 6: 5.000 cells, lane 7: 2.500 cells, lane 8: 1.000 cells
Product group Antibodies
OriGene
EGFR (Extracell. Dom.) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 20E12]AM00046PU-N
ApplicationsWestern Blot
ReactivityHuman
TargetEGFR
  • SizePrice
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