
IHC-P analysis of human colon using GTX88620 PU.1 antibody, Internal. Antigen retrieval : citrate buffer pH 6 Dilution : 3.75μg/ml
PU.1 antibody, Internal
GTX88620
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetSPI1
Overview
- SupplierGeneTex
- Product NamePU.1 antibody, Internal
- Delivery Days Customer7
- Application Supplier NoteWB: 0.03-0.1microg/ml. IHC-P: 3.75microg/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration0.50 mg/ml
- ConjugateUnconjugated
- Gene ID6688
- Target nameSPI1
- Target descriptionSpi-1 proto-oncogene
- Target synonymsAGM10, OF, PU.1, SFPI1, SPI-1, SPI-A, transcription factor PU.1, 31 kDa transforming protein, hematopoietic transcription factor PU.1, spleen focus forming virus (SFFV) proviral integration oncogene spi1
- HostGoat
- IsotypeIgG
- Protein IDP17947
- Protein NameTranscription factor PU.1
- Scientific DescriptionThis gene encodes an ETS-domain transcription factor that activates gene expression during myeloid and B-lymphoid cell development. The nuclear protein binds to a purine-rich sequence known as the PU-box found near the promoters of target genes, and regulates their expression in coordination with other transcription factors and cofactors. The protein can also regulate alternative splicing of target genes. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203






![WB analysis of THP-1 lysate using GTX17997 PU.1 antibody [PU1/2146].](https://www.genetex.com/upload/website/prouct_img/normal/GTX17997/GTX17997_20200115_WB_1889_w_23060620_196.webp)



![THP-1 whole cell and nuclear extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PU.1 antibody [N1C1] (GTX101581) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX101581/GTX101581_43600_20190607_WB_Fraction_w_23060100_464.webp)