![WB analysis of THP-1 lysate using GTX17997 PU.1 antibody [PU1/2146]. WB analysis of THP-1 lysate using GTX17997 PU.1 antibody [PU1/2146].](https://www.genetex.com/upload/website/prouct_img/normal/GTX17997/GTX17997_20200115_WB_1889_w_23060620_196.webp)
WB analysis of THP-1 lysate using GTX17997 PU.1 antibody [PU1/2146].
PU.1 antibody [PU1/2146]
GTX17997
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin, Other Application
Product group Antibodies
ReactivityHuman
TargetSPI1
Overview
- SupplierGeneTex
- Product NamePU.1 antibody [PU1/2146]
- Delivery Days Customer9
- Application Supplier NoteWB: 1-2microg/ml. ICC/IF: 1-2microg/ml. IHC-P: 1-2microg/ml for 30 minutes at RT. FCM: 1-2microg/106 cells. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin, Other Application
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDPU1/2146
- Concentration0.2 mg/ml
- ConjugateUnconjugated
- Gene ID6688
- Target nameSPI1
- Target descriptionSpi-1 proto-oncogene
- Target synonymsAGM10, OF, PU.1, SFPI1, SPI-1, SPI-A, transcription factor PU.1, 31 kDa transforming protein, hematopoietic transcription factor PU.1, spleen focus forming virus (SFFV) proviral integration oncogene spi1
- HostMouse
- IsotypeIgG2b
- Protein IDP17947
- Protein NameTranscription factor PU.1
- Scientific DescriptionThis gene encodes an ETS-domain transcription factor that activates gene expression during myeloid and B-lymphoid cell development. The nuclear protein binds to a purine-rich sequence known as the PU-box found near the promoters of target genes, and regulates their expression in coordination with other transcription factors and cofactors. The protein can also regulate alternative splicing of target genes. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC41116161
![IHC-P analysis of human lymph node tissue using GTX17997 PU.1 antibody [PU1/2146]. IHC-P analysis of human lymph node tissue using GTX17997 PU.1 antibody [PU1/2146].](https://www.genetex.com/upload/website/prouct_img/normal/GTX17997/GTX17997_20200115_IHC-P_717_w_23060620_868.webp)

![IHC-P analysis of human colon carcinoma tissue using GTX17997 PU.1 antibody [PU1/2146]. IHC-P analysis of human colon carcinoma tissue using GTX17997 PU.1 antibody [PU1/2146].](https://www.genetex.com/upload/website/prouct_img/normal/GTX17997/GTX17997_20200115_IHC-P_499_w_23060620_545.webp)
![IHC-P analysis of human spleen tissue using GTX17997 PU.1 antibody [PU1/2146]. IHC-P analysis of human spleen tissue using GTX17997 PU.1 antibody [PU1/2146].](https://www.genetex.com/upload/website/prouct_img/normal/GTX17997/GTX17997_20200115_IHC-P_1170_w_23060620_491.webp)
![FACS analysis of PFA-fixed Ramos cells using GTX17997 PU.1 antibody [PU1/2146]. Blue : Primary antibody Red : Isotype control FACS analysis of PFA-fixed Ramos cells using GTX17997 PU.1 antibody [PU1/2146]. Blue : Primary antibody Red : Isotype control](https://www.genetex.com/upload/website/prouct_img/normal/GTX17997/GTX17997_20200115_FACS_1755_w_23060620_945.webp)
![ICC/IF analysis of PFA-fixed Ramos cells using GTX17997 PU.1 antibody [PU1/2146]. Green : Primary antibody Red : Phalloidin ICC/IF analysis of PFA-fixed Ramos cells using GTX17997 PU.1 antibody [PU1/2146]. Green : Primary antibody Red : Phalloidin](https://www.genetex.com/upload/website/prouct_img/normal/GTX17997/GTX17997_20200115_ICC-IF_1756_w_23060620_971.webp)
![IHC-P analysis of human Hodgkin's lymphoma tissue using GTX17997 PU.1 antibody [PU1/2146]. IHC-P analysis of human Hodgkin's lymphoma tissue using GTX17997 PU.1 antibody [PU1/2146].](https://www.genetex.com/upload/website/prouct_img/normal/GTX17997/GTX17997_20200115_IHC-P_646_w_23060620_208.webp)







![THP-1 whole cell and nuclear extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PU.1 antibody [N1C1] (GTX101581) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX101581/GTX101581_43600_20190607_WB_Fraction_w_23060100_464.webp)

