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Rabbit anti-BMAL1 Antibody, Affinity Purified

A302-616A
Bethyl Laboratories
ApplicationsImmunoPrecipitation, Western Blot
Product group Antibodies
TargetARNTL
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Overview

  • Supplier
    Bethyl Laboratories
  • Product Name
    Rabbit anti-BMAL1 Antibody, Affinity Purified
  • Delivery Days Customer
    9
  • Applications
    ImmunoPrecipitation, Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1000 ug/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID406
  • Target name
    ARNTL
  • Target description
    aryl hydrocarbon receptor nuclear translocator like
  • Target synonyms
    ARNT-like protein 1, brain and muscle; aryl hydrocarbon receptor nuclear translocator-like protein 1; basic helix-loop-helix family member e5; basic-helix-loop-helix-PAS orphan MOP3; basic-helix-loop-helix-PAS protein MOP3; bHLHe5; bHLH-PAS protein JAP3; BMAL1; BMAL1c; brain and muscle ARNT-like 1; class E basic helix-loop-helix protein 5; JAP3; member of PAS protein 3; member of PAS superfamily 3; MOP3; PAS domain containing 3; PAS domain-containing protein 3; PASD3; testis tissue sperm-binding protein Li 50e; TIC
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO00327
  • Protein Name
    Aryl hydrocarbon receptor nuclear translocator-like protein 1
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of BMAL1/ARNTL using anti-BMAL1/ARNTL antibody (A00260-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HT1080 whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human SH-SY5Y whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat liver tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BMAL1/ARNTL antigen affinity purified polyclonal antibody (Catalog # A00260-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BMAL1/ARNTL at approximately 75 kDa. The expected band size for BMAL1/ARNTL is at 69 kDa.
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