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Rabbit anti-POGZ IHC Antibody, Affinity Purified

IHC-00712
Bethyl Laboratories
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityAvian, Bovine, Canine, Equine, Hamster, Human, Mammals, Monkey, Mouse, Opossum, Porcine, Primate, Rabbit, Rat
TargetPOGZ

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Overview

  • Supplier
    Bethyl Laboratories
  • Product Name
    Rabbit anti-POGZ IHC Antibody, Affinity Purified
  • Delivery Days Customer
    9
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    250 ug/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID23126
  • Target name
    POGZ
  • Target description
    pogo transposable element derived with ZNF domain
  • Target synonyms
    MRD37, WHSUS, ZNF280E, ZNF635, ZNF635m, pogo transposable element with ZNF domain, putative protein product of Nbla00003, zinc finger protein 280E, zinc finger protein 635
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ7Z3K3
  • Protein Name
    Pogo transposable element with ZNF domain
  • Reactivity
    Avian, Bovine, Canine, Equine, Hamster, Human, Mammals, Monkey, Mouse, Opossum, Porcine, Primate, Rabbit, Rat
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of POGZ using anti-POGZ antibody (A06169-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-POGZ antigen affinity purified polyclonal antibody (Catalog # A06169-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for POGZ at approximately 220 kDa. The expected band size for POGZ is at 155 kDa.
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