Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with RIP antibody [C1C3] (GTX111235) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
![RIP antibody [C1C3] detects RIP protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded human cervical carcinoma. RIP stained by RIP antibody [C1C3] (GTX111235) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX111235/GTX111235_44314_20220325_IHC-P_w_23060500_558.webp "RIP antibody [C1C3] detects RIP protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded human cervical carcinoma. RIP stained by RIP antibody [C1C3] (GTX111235) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with RIP antibody [C1C3] (GTX111235) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX111235/GTX111235_44412_20210903_WB_w_23060500_853.webp "Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with RIP antibody [C1C3] (GTX111235) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with RIP antibody [C1C3] (GTX111235) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
RIP antibody [C1C3]
GTX111235
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetRIPK1
Overview
- SupplierGeneTex
- Product NameRIP antibody [C1C3]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1.06 mg/ml
- ConjugateUnconjugated
- Gene ID8737
- Target nameRIPK1
- Target descriptionreceptor interacting serine/threonine kinase 1
- Target synonymsAIEFL, IMD57, RIP, RIP-1, RIP1, receptor-interacting serine/threonine-protein kinase 1, cell death protein RIP, receptor (TNFRSF)-interacting serine-threonine kinase 1, receptor-interacting protein 1, receptor-interacting protein kinase 1, serine/threonine-protein kinase RIP
- HostRabbit
- IsotypeIgG
- Protein IDQ13546
- Protein NameReceptor-interacting serine/threonine-protein kinase 1
- Scientific DescriptionEssential adapter molecule for the activation of NF-kappa-B. Following different upstream signals (binding of inflammatory cytokines, stimulation of pathogen recognition receptors, or DNA damage), particular RIPK1-containing complexes are formed, initiating a limited number of cellular responses. Upon TNFA stimulation RIPK1 is recruited to a TRADD-TRAF complex initiated by TNFR1 trimerization. There, it is ubiquitinated via Lys-63-link chains, inducing its association with the IKK complex, and its activation through NEMO binding of polyubiquitin chains.
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
Datasheet
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