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Anti-RIPK1 Antibody

144-66389
RayBiotech
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
Product group Antibodies
TargetRIPK1
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-RIPK1 Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Conjugate
    Unconjugated
  • Gene ID8737
  • Target name
    RIPK1
  • Target description
    receptor interacting serine/threonine kinase 1
  • Target synonyms
    AIEFL, IMD57, RIP, RIP-1, RIP1, receptor-interacting serine/threonine-protein kinase 1, cell death protein RIP, receptor (TNFRSF)-interacting serine-threonine kinase 1, receptor-interacting protein 1, receptor-interacting protein kinase 1, serine/threonine-protein kinase RIP
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ13546
  • Protein Name
    Receptor-interacting serine/threonine-protein kinase 1
  • Scientific Description
    RIPK1 pAb
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Western Blot Positive WB detected in: NIH/3T3 whole cell lysate, Hela whole cell lysate, K562 whole cell lysate, Jurkat whole cell lysate, 293 whole cell lysate, Rat liver tissue, Mouse heart tissue, Mouse kidney tissue All lanes: RIPK1 antibody at 3.2microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 76, 71 kDa Observed band size: 76 kDa
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Lane 1: Raji cell lysates probed with RIPK-1 Polyclonal Antibody, Unconjugated (bs-5805R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
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Figure 1. Western blot analysis of RIP using anti-RIP antibody (PB9116). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: recombinant human RIP protein 0.5 ng. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RIP antigen affinity purified polyclonal antibody (Catalog # PB9116) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RIP at approximately 38 kDa. The expected band size for RIP is at 38 kDa.
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