RIP antibody [HL2250] detects RIP protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. RIP stained by RIP antibody [HL2250] (GTX638299) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
![Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with RIP antibody [HL2250] (GTX638299) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX638299/GTX638299_45026_20230428_WB_23050223_780.webp "Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with RIP antibody [HL2250] (GTX638299) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![Untreated (–) and treated (+) NCI-H929 whole cell extract (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with RIP antibody [HL2250] (GTX638299) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](https://www.genetex.com/upload/website/prouct_img/normal/GTX638299/GTX638299_45026_20230707_WB_treatment_Melarsoprol_23071223_681.webp "Untreated (–) and treated (+) NCI-H929 whole cell extract (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with RIP antibody [HL2250] (GTX638299) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
![RIP antibody [HL2250] detects RIP protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: RIP stained by RIP antibody [HL2250] (GTX638299) diluted at 1:100. Blue: Fluoroshield with DAPI (GTX30920).](https://www.genetex.com/upload/website/prouct_img/normal/GTX638299/GTX638299_45026_20230721_ICC_IF_23072519_552.webp "RIP antibody [HL2250] detects RIP protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: RIP stained by RIP antibody [HL2250] (GTX638299) diluted at 1:100. Blue: Fluoroshield with DAPI (GTX30920).")
RIP antibody [HL2250] detects RIP protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. RIP stained by RIP antibody [HL2250] (GTX638299) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
RIP antibody [HL2250]
GTX638299
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetRIPK1
Overview
- SupplierGeneTex
- Product NameRIP antibody [HL2250]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDHL2250
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID8737
- Target nameRIPK1
- Target descriptionreceptor interacting serine/threonine kinase 1
- Target synonymsAIEFL, IMD57, RIP, RIP-1, RIP1, receptor-interacting serine/threonine-protein kinase 1, cell death protein RIP, receptor (TNFRSF)-interacting serine-threonine kinase 1, receptor-interacting protein 1, receptor-interacting protein kinase 1, serine/threonine-protein kinase RIP
- HostRabbit
- IsotypeIgG
- Protein IDQ13546
- Protein NameReceptor-interacting serine/threonine-protein kinase 1
- Scientific DescriptionThis gene encodes a member of the receptor-interacting protein (RIP) family of serine/threonine protein kinases. The encoded protein plays a role in inflammation and cell death in response to tissue damage, pathogen recognition, and as part of developmental regulation. RIPK1/RIPK3 kinase-mediated necrosis is referred to as necroptosis. Genetic disruption of this gene in mice results in death shortly after birth. [provided by RefSeq, Aug 2017]
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
Datasheet
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