
Immunofluorescence analysis of methanol-fixed HeLa, using RIP(GTX111074) antibody at 1:100 dilution.
RIP antibody
GTX111074
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetRIPK1
Overview
- SupplierGeneTex
- Product NameRIP antibody
- Delivery Days Customer9
- Application Supplier NoteWB: 1:1000-1:10000. ICC/IF: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID8737
- Target nameRIPK1
- Target descriptionreceptor interacting serine/threonine kinase 1
- Target synonymsAIEFL, IMD57, RIP, RIP-1, RIP1, receptor-interacting serine/threonine-protein kinase 1, cell death protein RIP, receptor (TNFRSF)-interacting serine-threonine kinase 1, receptor-interacting protein 1, receptor-interacting protein kinase 1, serine/threonine-protein kinase RIP
- HostRabbit
- IsotypeIgG
- Protein IDQ13546
- Protein NameReceptor-interacting serine/threonine-protein kinase 1
- Scientific DescriptionEssential adapter molecule for the activation of NF-kappa-B. Following different upstream signals (binding of inflammatory cytokines, stimulation of pathogen recognition receptors, or DNA damage), particular RIPK1-containing complexes are formed, initiating a limited number of cellular responses. Upon TNFA stimulation RIPK1 is recruited to a TRADD-TRAF complex initiated by TNFR1 trimerization. There, it is ubiquitinated via Lys-63-link chains, inducing its association with the IKK complex, and its activation through NEMO binding of polyubiquitin chains.
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC41116161






![RIP antibody [HL2249] detects RIP protein at endoplasmic reticulum and cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: RIP stained by RIP antibody [HL2249] (GTX638298) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).](https://www.genetex.com/upload/website/prouct_img/normal/GTX638298/GTX638298_T-44960_20230331_ICC_IF_23041023_489.webp)
![RIP antibody [HL2250] detects RIP protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. RIP stained by RIP antibody [HL2250] (GTX638299) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX638299/GTX638299_T-44960_20230325_IHC-P_23032819_534.webp)
![Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with RIP antibody [GT1168] (GTX09128) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX09128/GTX09128_40000000059_20200306_WB_w_23053123_930.webp)