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Search results: ChIP

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Product group Antibodies
References
GeneTex
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ChIP Chromatin ImmunoPrecipitation, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman
TargetBRCA1
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Anti-RIP140 Antibody ChIP grade
Product group Antibodies
Antibodies.com
ApplicationsImmunoFluorescence, Western Blot, ChIP Chromatin ImmunoPrecipitation, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, Other Application
ReactivityChicken, Human, Mouse, Rat
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ChIP analysis of sheared chromatin from 10? HeLa cells treated with colcemid using GTX60327 Histone H3K9me3S10ph (Tri-methyl Lys9/phospho Ser10) antibody - ChIP grade. A titration of the antibody consisting of 1, 5, and 10 microl per ChIP experiment was analysed. Additionally, the same titration was analysed after incubation of the antibody with 5 nmol blocking peptide for 1 hour at room temperature. IgG (5 microg/IP) was used as negative IP control. QPCR was performed with primers for the promoter of the active genes GAPDH and c-fos and for the heterochromatin marker Sat2. This figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).
Product group Antibodies
GeneTex
ApplicationsDot Blot, ImmunoFluorescence, Western Blot, ChIP Chromatin ImmunoPrecipitation, ELISA, ImmunoCytoChemistry
ReactivityHuman
TargetH3C1
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ChIP analysis of sheared chromatin from 10? HeLa cells treated with colcemid using GTX60329 Histone H3K27me3S28ph (Tri-methyl Lys27/phospho Ser28) antibody - ChIP grade. A titration of the antibody consisting of 1, 5 and 10 microl per ChIP experiment was analysed. Additionally, ChIP was performed after incubation of the antibody with 5 nmol blocking peptide for 1 hour at room temperature. IgG (5 microg/IP) was used as negative IP control. QPCR was performed with primers for the promoters of the active genes c-fos and RPL30 and for the inactive gene MYOD. This figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).
Product group Antibodies
GeneTex
ApplicationsDot Blot, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ChIP Chromatin ImmunoPrecipitation, ELISA, ImmunoCytoChemistry
ReactivityHuman
TargetH3C1
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ChIP analysis of sheared chromatin from 10? HeLa cells using GTX60334 Histone H4K5K8K12K16ac (acetyl Lys5/Lys8/Lys12/Lys16) antibody - ChIP grade. A titration of the antibody consisting of 1, 2, 5 and 10 microg per ChIP experiment was analysed. IgG (2 microg/IP) was used as negative IP control. QPCR was performed with primers for promoter of the active gene EIF4A2 and for a region 1 kb upstream of the GAPDH gene, used as positive controls, and for the inactive MYOD1 gene and the Sat2 satellite repeat region used as negative controls. This figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).
Product group Antibodies
GeneTex
ApplicationsDot Blot, ImmunoFluorescence, ChIP Chromatin ImmunoPrecipitation, ELISA, ImmunoCytoChemistry
ReactivityHuman, Mouse
TargetH4C1
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Product group Antibodies
GeneTex
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ChIP analysis of sheared chromatin from 10? HeLa cells using GTX60825 Histone H3R17me2K18ac (Asymmetric Di-methyl Arg17/Acetyl Lys18) antibody - ChIP grade. A titration consisting of 1, 2, 5 and 10 microg of antibody per ChIP experiment was analyzed. IgG (2 microg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the promoters of the active EIF4A2 and c-fos genes, used as positive controls and for the inactive MYOD1 gene and the Sat2 satellite repeat, used as negative controls. This figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).
Product group Antibodies
GeneTex
ApplicationsDot Blot, ImmunoFluorescence, Western Blot, ChIP Chromatin ImmunoPrecipitation, ELISA, ImmunoCytoChemistry
ReactivityHuman, Mouse
TargetH3C1
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Product group Antibodies
RayBiotech
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Product group Technique Specific Products
Santa Cruz Biotechnology
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Product group Technique Specific Products
Santa Cruz Biotechnology
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Product group Technique Specific Products
Santa Cruz Biotechnology
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Product group Technique Specific Products
Santa Cruz Biotechnology
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