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Search results: ChIP

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ChIP analysis of sheared chromatin from 10? HeLa cells using GTX60815 Histone H3K27ac (Acetyl Lys27) antibody - ChIP grade. A titration consisting of 0.2, 0.5, 1 and 2 microg of antibody per ChIP experiment was analyzed. IgG (1 microg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the promoters of the active GAPDH and EIF4A2 genes, used as positive controls, and for the coding regions of the inactive MB and MYT1 genes, used as negative controls. This figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).
Product group Antibodies
References
GeneTex
Histone H3K27ac (Acetyl Lys27) antibody - ChIP gradeGTX60815
ApplicationsDot Blot, ImmunoFluorescence, Western Blot, ChIP Chromatin ImmunoPrecipitation, ELISA, ImmunoCytoChemistry
ReactivityHuman, Mouse, Plant, Rat
  • SizePrice
ChIP analysis of sheared chromatin from 10? HeLa cells treated with colcemid using GTX60827 Histone H3S10ph (phospho Ser10) antibody - ChIP grade. A titration of the antibody consisting of 1, 5, and 10 microl per ChIP experiment was analysed. Additionally, ChIP was performed after incubation of the antibody with 5 nmol blocking peptide for 1 hour at room temperature. IgG (5 microg/IP) was used as negative IP control. QPCR was performed with primers for the promoter of the active genes c-fos and RPL30. This figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).
Product group Antibodies
GeneTex
Histone H3S10ph (phospho Ser10) antibody - ChIP gradeGTX60827
ApplicationsDot Blot, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ChIP Chromatin ImmunoPrecipitation, ELISA, ImmunoCytoChemistry
ReactivityHuman
TargetH3C1
  • SizePrice
Product group Antibodies
GeneTex
Histone H4K20me3 (trimethyl Lys20) antibody - ChIP gradeGTX60828
ApplicationsDot Blot, ImmunoFluorescence, Western Blot, ChIP Chromatin ImmunoPrecipitation, ELISA, ImmunoCytoChemistry, Other Application
ReactivityHuman, Mouse
TargetH4C9
  • SizePrice
ChIP analysis of sheared chromatin from 10? HeLa cells using GTX60829 Histone H4K8ac (acetyl Lys8) antibody - ChIP grade. A titration of the antibody consisting of 1, 2, 5, and 10 microg per ChIP experiment was analysed. IgG (1 microg/IP) was used as negative IP control. QPCR was performed with primers for the c-fos promoter and for the Sat2 satellite repeat region. This figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).
Product group Antibodies
GeneTex
Histone H4K8ac (acetyl Lys8) antibody - ChIP gradeGTX60829
ApplicationsDot Blot, ImmunoFluorescence, Western Blot, ChIP Chromatin ImmunoPrecipitation, ELISA, ImmunoCytoChemistry
ReactivityHuman, Mouse
TargetH4C9
  • SizePrice
ChIP analysis of sheared chromatin from 10? HeLaS3 cells using GTX60336 Histone H3K79me3 (Tri-methyl Lys79) antibody - ChIP grade. A titration of the antibody consisting of 1, 2, 5 and 10 microg per ChIP experiment was analysed. IgG (5 microg/IP) was used as negative IP control. Quantitative PCR was performed with primers for the GAPDH promoter and for exon 2 of the inactive myoglobin gene. This figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). These results are in accordance with the observation that H3K79me3 shows a preference for active promoters.
Product group Antibodies
GeneTex
Histone H3K79me3 (Tri-methyl Lys79) antibody - ChIP gradeGTX60336
ApplicationsDot Blot, Western Blot, ChIP Chromatin ImmunoPrecipitation, ELISA
ReactivityHuman, Mouse
TargetH3C1
  • SizePrice
ChIP analysis of sheared chromatin from 5x10? K562 cells using GTX60350 Histone H3K27me1 (Mono-methyl Lys27) antibody - ChIP grade. A titration of the antibody consisting of 0.5, 1, 2 and 5 microg per ChIP experiment was analysed. IgG (2 microg/IP) was used as negative IP control. Figure 1A. Quantitative PCR was performed with primers for the coding sequence of the active GAPDH and ACTB genes, used as positive controls, and for the inactive MYOD1 and MYT1 genes, used as negative controls. The graph shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). Figure 1B. Recovery of the nucleosomes carrying the H3K27me1, H3K27me2, H3K27me3, H3K4me1, H3K9me1, H3K36me1, H4K20me1 and the unmodified H3K4 as determined by qPCR. The figure clearly shows the antibody is specific in ChIP for the H3K27me1 modification with some slight cross reaction with H3K36me1 and H3K9me1 at higher concentrations.
Product group Antibodies
GeneTex
Histone H3K27me1 (Mono-methyl Lys27) antibody - ChIP gradeGTX60350
ApplicationsDot Blot, ImmunoFluorescence, Western Blot, ChIP Chromatin ImmunoPrecipitation, ELISA, ImmunoCytoChemistry
ReactivityHuman, Plant
TargetH3C1
  • SizePrice
ChIP analysis of sheared chromatin from 10? HeLa cells using GTX60351 Histone H3K27me2 (Di-methyl Lys27) antibody - ChIP grade. A titration of the antibody consisting of 1, 2, 5, and 10 microg per ChIP experiment was analysed. IgG (2 microg/IP) was used as negative IP control. QPCR was performed with primers for the promoter of the active GAPDH and EIF4A2 genes, used as negative controls, and for the promoter of the inactive HBB and the coding region of the inactive MYOD1 genes, used as positive controls. This figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). These results are in accordance with the observation that H3K27me2 is preferably present at silent genes.
Product group Antibodies
GeneTex
Histone H3K27me2 (Di-methyl Lys27) antibody - ChIP gradeGTX60351
ApplicationsDot Blot, ImmunoFluorescence, Western Blot, ChIP Chromatin ImmunoPrecipitation, ELISA, ImmunoCytoChemistry
ReactivityHuman, Zebra Fish
TargetH3C1
  • SizePrice
ChIP analysis of sheared chromatin from 10? HeLa cells using GTX60352 Histone H3K9me1 (Mono-methyl Lys9) antibody - ChIP grade. Respectively 1 and 5 microg of the antibody and 5 microg of IgG (negative IP control) were used per ChIP experiment. QPCR was performed with primers for the GAPDH promoter and for the inactive gene MYOD. This figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). These results are in accordance with the observation that H3K9me1 is preferably present at silent genes.
Product group Antibodies
GeneTex
Histone H3K9me1 (Mono-methyl Lys9) antibody - ChIP gradeGTX60352
ApplicationsDot Blot, Western Blot, ChIP Chromatin ImmunoPrecipitation, ELISA
ReactivityHuman, Other Species
TargetH3C1
  • SizePrice
ChIP analysis of sheared chromatin from 10? HeLa cells using GTX60353 Histone H3K79me1 (Mono-methyl Lys79) antibody - ChIP grade. A titration consisting of 1, 2, 5 and 10 microg of antibody per ChIP experiment was analyzed. IgG (2 microg/IP) was used as a negative IP control. Quantitative PCR was performed with primers specific for a region 1 kb upstream of the promoter and the coding region of the active GAPDH gene, used as positive controls, and for exon 2 of the inactive myoglobin (MB) gene and the Sat2 satellite repeat, used as negative controls. This figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).
Product group Antibodies
GeneTex
Histone H3K79me1 (Mono-methyl Lys79) antibody - ChIP gradeGTX60353
ApplicationsDot Blot, Western Blot, ChIP Chromatin ImmunoPrecipitation, ELISA
ReactivityHuman, Yeast
TargetH3C1
  • SizePrice
ChIP analysis of sheared chromatin from 1.5x10? U2OS cells using GTX60354 Histone H3K36me1 (Mono-methyl Lys36) antibody - ChIP grade. A titration consisting of 1, 2, 5 and 10 microg of antibody per ChIP experiment was analyzed. IgG (1 microg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the promoter of the constitutively expressed GAPDH gene and for the inactive gene MYOD. This figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).
Product group Antibodies
GeneTex
Histone H3K36me1 (Mono-methyl Lys36) antibody - ChIP gradeGTX60354
ApplicationsDot Blot, ImmunoFluorescence, Western Blot, ChIP Chromatin ImmunoPrecipitation, ELISA, ImmunoCytoChemistry
ReactivityHuman
TargetH3C1
  • SizePrice
ChIP analysis of sheared chromatin from 1.6x10? U2OS cells using GTX60356 Histone H3K9me1 (Mono-methyl Lys9) antibody - ChIP grade. A titration of the antibody consisting of 2, 5, 10 and 15 microl per ChIP experiment was analysed. IgG (5 microg/IP) was used as negative IP control. Quantitative PCR was performed using primers for the promoter of the housekeeping gene GAPDH and for the coding region of the myogenic differentiation gene (MYOD), a gene that is inactive at normal conditions. This figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).
Product group Antibodies
GeneTex
Histone H3K9me1 (Mono-methyl Lys9) antibody - ChIP gradeGTX60356
ApplicationsDot Blot, ImmunoFluorescence, Western Blot, ChIP Chromatin ImmunoPrecipitation, ELISA, ImmunoCytoChemistry
ReactivityHuman
TargetH3C1
  • SizePrice
ChIP analysis of sheared chromatin from 10? HeLa cells using GTX60357 Histone H3K9me2 (Di-methyl Lys9) antibody - ChIP grade. A titration of the antibody consisting of 1, 2, 5, and 10 microg per ChIP experiment was analysed. IgG (2 microg/IP) was used as negative IP control. QPCR was performed with primers specific for promoter of the inactive HBB gene and the coding region of the inactive MYOD gene, used as positive controls, and for the promoters of the active genes c-fos and GAPDH, used as negative controls. This figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).
Product group Antibodies
GeneTex
Histone H3K9me2 (Di-methyl Lys9) antibody - ChIP gradeGTX60357
ApplicationsDot Blot, ImmunoFluorescence, Western Blot, ChIP Chromatin ImmunoPrecipitation, ELISA, ImmunoCytoChemistry
ReactivityHuman, Mouse, Plant, Xenopus
TargetH3C1
  • SizePrice
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