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Search results: DHFR

49 to 60 of 593 products
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Product group Antibodies
MyBioSource
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Product group Antibodies
MyBioSource
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Product group Antibodies
MyBioSource
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GTX110710-pro Image
Product group Molecular Biology
GeneTex
Human DHFR proteinGTX110710-PRO
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GTX57632 WB Image
Product group Antibodies
GeneTex
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
ReactivityHuman
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Product group Antibodies
Biorbyt
DHFR antibodyORB626366
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetDHFR
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DHFR-IN-5
Product group Chemicals
TargetMol Chemicals
DHFR-IN-5T73328
CAS Number1142407-81-5
Molecular Weight360.41
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DHFR-IN-4
Product group Chemicals
References
TargetMol Chemicals
DHFR-IN-4T61486
CAS Number2820126-49-4
Molecular Weight371.46
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DHFR-IN-3
Product group Chemicals
References
TargetMol Chemicals
CAS Number137553-43-6
Molecular Weight239.07
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DHFR-IN-2
Product group Chemicals
TargetMol Chemicals
DHFR-IN-2T60286
CAS Number331942-46-2
Molecular Weight227.26
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DHFR-IN-1
Product group Chemicals
TargetMol Chemicals
DHFR-IN-1T63379
CAS Number2757991-65-2
Molecular Weight498.58
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Western blot analysis of DHFR using anti-DHFR antibody (M00813) in (1) HeLa cell lysate; (2) Mouse spleen lysate. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DHFR antigen affinity purified polyclonal antibody (Catalog # M00813) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system.
Product group Antibodies
Boster Bio