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Figure 1. Western blot analysis of Amphiregulin using anti-Amphiregulin antibody (A01787-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Amphiregulin antigen affinity purified polyclonal antibody (Catalog # A01787-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Amphiregulin at approximately 41KD. The expected band size for Amphiregulin is at 28KD.
Product group Antibodies
Boster Bio
Anti-Amphiregulin/Areg Antibody Picoband(r)A01787-1-CY3
ApplicationsFlow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry
TargetAreg
  • SizePrice
Figure 1. Western blot analysis of Amphiregulin using anti-Amphiregulin antibody (A01787-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Amphiregulin antigen affinity purified polyclonal antibody (Catalog # A01787-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Amphiregulin at approximately 41KD. The expected band size for Amphiregulin is at 28KD.
Product group Antibodies
Boster Bio
Anti-Amphiregulin/Areg Antibody Picoband(r)A01787-1-DYLIGHT488
ApplicationsFlow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry
TargetAreg
  • SizePrice
Figure 1. Western blot analysis of Amphiregulin using anti-Amphiregulin antibody (A01787-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Amphiregulin antigen affinity purified polyclonal antibody (Catalog # A01787-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Amphiregulin at approximately 41KD. The expected band size for Amphiregulin is at 28KD.
Product group Antibodies
Boster Bio
Anti-Amphiregulin/Areg Antibody Picoband(r)A01787-1-DYLIGHT550
ApplicationsFlow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry
TargetAreg
  • SizePrice
Figure 1. Western blot analysis of Amphiregulin using anti-Amphiregulin antibody (A01787-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Amphiregulin antigen affinity purified polyclonal antibody (Catalog # A01787-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Amphiregulin at approximately 41KD. The expected band size for Amphiregulin is at 28KD.
Product group Antibodies
Boster Bio
Anti-Amphiregulin/Areg Antibody Picoband(r)A01787-1-DYLIGHT594
ApplicationsFlow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry
TargetAreg
  • SizePrice
Figure 1. Western blot analysis of Amphiregulin using anti-Amphiregulin antibody (A01787-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Amphiregulin antigen affinity purified polyclonal antibody (Catalog # A01787-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Amphiregulin at approximately 41KD. The expected band size for Amphiregulin is at 28KD.
Product group Antibodies
Boster Bio
Anti-Amphiregulin/Areg Antibody Picoband(r)A01787-1-FITC
ApplicationsFlow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry
TargetAreg
  • SizePrice
Figure 1. Western blot analysis of Amphiregulin using anti-Amphiregulin antibody (A01787-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Amphiregulin antigen affinity purified polyclonal antibody (Catalog # A01787-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Amphiregulin at approximately 41KD. The expected band size for Amphiregulin is at 28KD.
Product group Antibodies
Boster Bio
Anti-Amphiregulin/Areg Antibody Picoband(r)A01787-1-HRP
ApplicationsFlow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry
TargetAreg
  • SizePrice
Figure 1. Western blot analysis of Amphiregulin using anti-Amphiregulin antibody (A01787-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Amphiregulin antigen affinity purified polyclonal antibody (Catalog # A01787-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Amphiregulin at approximately 41KD. The expected band size for Amphiregulin is at 28KD.
Product group Antibodies
Boster Bio
Anti-Amphiregulin/Areg Antibody Picoband(r)A01787-1-IFLUOR647
ApplicationsFlow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry
TargetAreg
  • SizePrice
Figure 1. Western blot analysis of Amphiregulin using anti-Amphiregulin antibody (A01787-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Amphiregulin antigen affinity purified polyclonal antibody (Catalog # A01787-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Amphiregulin at approximately 41KD. The expected band size for Amphiregulin is at 28KD.
Product group Antibodies
Boster Bio
Anti-Amphiregulin/Areg Antibody Picoband(r)A01787-1-PE
ApplicationsFlow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry
TargetAreg
  • SizePrice
Figure 1. Western blot analysis of Amphiregulin using anti-Amphiregulin antibody (A01787-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Amphiregulin antigen affinity purified polyclonal antibody (Catalog # A01787-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Amphiregulin at approximately 41KD. The expected band size for Amphiregulin is at 28KD.
Product group Antibodies
Boster Bio
Anti-Amphiregulin/Areg Antibody Picoband(r)A01787-1
ApplicationsFlow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry
TargetAreg
  • SizePrice
Product group Antibodies
Biorbyt
Anti-CCDC109A AntibodyORB737311
ApplicationsWestern Blot, ImmunoHistoChemistry
TargetMCU
  • SizePrice
Product group Antibodies
Cusabio
MCUB Antibody, HRP conjugatedCSB-PA873671LB01HU
ApplicationsELISA
TargetMCUB
  • SizePrice
Product group Antibodies
Cusabio
MCUB Antibody, FITC conjugatedCSB-PA873671LC01HU
TargetMCUB
  • SizePrice
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