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Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with AKT3 antibody (GTX113312) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Product group Antibodies
References
GeneTex
AKT3 antibodyGTX113312
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman, Mouse
TargetAKT3
  • SizePrice
Akt1 antibody immunoprecipitates Akt1 protein in IP experiments. IP samples: 30 μg whole cell extract of Akt1-transfected 293T cells. A. 30 μg whole cell extract of Akt1-protein expressing 293T cell B. Control with 3 μg of preimmune Rabbit IgG C. Immunoprecipitation of Akt1 protein by 3 μg Akt1 antibody (GTX110613) 10 % SDS-PAGE The immunoprecipitated Akt1 protein was detected by Akt1 antibody (GTX110613) diluted at 1:5000. [EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
Product group Antibodies
References
GeneTex
AKT1 antibodyGTX110613
ApplicationsImmunoPrecipitation, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman, Mouse
TargetAKT1
  • SizePrice
Untreated (–) and treated (+) Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AKT1 (phospho Ser129) antibody (GTX134836) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Product group Antibodies
GeneTex
ApplicationsWestern Blot
ReactivityHuman, Rat
TargetAKT1
  • SizePrice
WB analysis of human kidney tissue lysate using GTX13919 AKT3 antibody [66C1247]. Dilution : 2 μg/ml Loading : 30μg
Product group Antibodies
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
ReactivityHuman, Mouse, Rat
TargetAKT3
  • SizePrice
ICC/IF analysis of U2OS cells using GTX16378 TCL1A antibody. Blue : DAPI
Product group Antibodies
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
ReactivityHuman
TargetTCL1A
  • SizePrice
IHC-P analysis of human spleen tissue using GTX17841 TCL1A antibody [TCL1/2078].
Product group Antibodies
GeneTex
ApplicationsImmunoHistoChemistry, ImmunoHistoChemistry Paraffin, Other Application
ReactivityHuman
TargetTCL1A
  • SizePrice
Analysis of Protein Array containing more than 19,000 full-length human proteins using AKT1 Mouse Monoclonal Antibody (AKT1/2552). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target if the Monoclonal Antibody has an S-score of at least 2.5. For example, if a Monoclonal Antibody binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that Monoclonal Antibody to protein X is equal to 29.
Product group Antibodies
GeneTex
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin, Other Application
ReactivityHuman
TargetAKT1
  • SizePrice
IHC-P analysis of human renal cell carcinoma tissue using GTX17980 PTEN antibody [PTEN/2110].
Product group Antibodies
GeneTex
ApplicationsImmunoHistoChemistry, ImmunoHistoChemistry Paraffin, Other Application
ReactivityHuman
TargetPTEN
  • SizePrice
WB analysis of various sample lysates using GTX18153 PTEN antibody. Dilution : 1:1000 Loading : 25μg per lane
Product group Antibodies
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman, Mouse, Rat
TargetPTEN
  • SizePrice
Western blot using GeneTex's affinity purified anti-PDK-1 antibody shows detection of myc-tagged human PDK-1 at 60kDa in ~10 μg of a virus infected Sf9 cell lysate (arrow). The nitrocellulose membrane was probed overnight at 4o C with the primary antibody diluted 1:750 in 1% non-fat dry milk. HRP conjugated Goat-anti-Rabbit IgG [H&L] and chemiluminescent detection is recommended. Other detection systems will yield similar results.
Product group Antibodies
GeneTex
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman
TargetPDK1
  • SizePrice
Product group Antibodies
GeneTex
FTS antibodyGTX30041
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman, Mouse, Rat
TargetAKTIP
  • SizePrice
WB analysis of rat liver tissue lysate using GTX31300 sRANKL antibody. Working concentration : (A) 0.25 and (B) 0.5 μg/ml
Product group Antibodies
GeneTex
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman, Mouse, Rat
TargetTNFSF11
  • SizePrice

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