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Immunofluorescence Microscopy of Mouse Anti-AKTpS473 antibody using STED nanoscopy to evaluate AKT activation and migration. Tissue: A431 cells. Antigen retrieval: Panel A: serum starved,unstimulated cells. Panel B: serum starved, EGF stimulated for 15 mins. A massive increase in AKT-pS473 activation, as measured by intensity signal, peaked at 15 minutes and was associated with depolymerized tubulin. Staining: Panel A shows STED data (AKT-pS473, red channel) collected simultaneously with confocal signal (a-tubulin, green channel). Upon stimulation of cells with EGF, a rapid activation of AKT is observed (Panel B) along with a coincident change in the tubulin organization (yellow signal), as well as an extensive cell shape-change (cell membrane folding) and accumulation of AKTpS473 at the cell periphery.
Product group Antibodies
OriGene
AKT1 Mouse Monoclonal Antibody [Clone ID: 17F6.B11]TA396764
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse
TargetAKT1
  • SizePrice
Immunohistochemistry of Mouse anti-AKT pT308 antibody. Tissue: human brain cerebellum tissue (40X). Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: AKT pT308 antibody at 20 µg/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: staining of Purkinje neurons and cell processes in the cerebellum, cytosolic as well as occasionally nuclear. Staining: AKT pT308 as precipitated red signal with hematoxylin purple nuclear counterstain.
Product group Antibodies
OriGene
AKT1 Mouse Monoclonal Antibody [Clone ID: 18F3.H11]TA396765
ApplicationsFlow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse
TargetAKT1
  • SizePrice
Western Blot of Mouse anti-AKT antibody. Lane 1: unstimulated NIH/3T3 cell lysates (p/n W10-000-358). Lane 2: PDGF stimulated NIH/3T3 cell lysates (p/n W10-001-377). Load: 10 µg per lane. Primary antibody: AKT antibody at 1:400 for overnight at 4°C. Secondary antibody: HRP conjugated Gt-a-Mouse IgG (p/n 610-103-121) was used at a 1:40,000 dilution for 1 h at 4° C with FemtoMax™ enhanced chemiluminescent reagent (p/n FEMTOMAX-100). Block: 5% BLOTTO (p/n B501-0500) in TBS for 2h at RT. Observed size: ~56 kDa for AKT. Other band(s): none.
Product group Antibodies
OriGene
AKT1 Mouse Monoclonal Antibody [Clone ID: 14E5.16C8.25F6]TA396766
ApplicationsFlow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityMouse
TargetAKT1
  • SizePrice
Product group Antibodies
OriGene
Rabbit Monoclonal Antibody [Clone ID: RM251]TA398436
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetAKT1
  • SizePrice
Product group Antibodies
OriGene
AKT1 Rabbit Monoclonal Antibody [Clone ID: RM316]TA398513
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman
TargetAKT1
  • SizePrice
Western blot analysis of various lysates using Pan-Akt Rabbit mAb (TA420004) at1:2000 dilution.<br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at1:10000 dilution.<br/>Lysates/proteins: 25μg per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit .<br/>Exposure time: 10s.
Product group Antibodies
OriGene
AKT1 Rabbit Polyclonal AntibodyTA420004
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetAKT1
  • SizePrice
Western blot analysis of various lysates using Phospho-Akt-S473 Rabbit mAb (TA420035) at 1:1000 dilution. Both Jurkat and C6 cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.<br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25μg per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit .<br/>Exposure time: 1s.
Product group Antibodies
OriGene
Phospho-AKT1 Rabbit Polyclonal AntibodyTA420035
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetAKT1
  • SizePrice
Western blot analysis of various lysates using Phospho-Akt-T308 Rabbit mAb (TA420036) at 1:1000 dilution. NIH/3T3 and C6 cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.<br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25μg per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit .<br/>Exposure time: 180s.
Product group Antibodies
OriGene
Phospho-AKT1 Rabbit Polyclonal AntibodyTA420036
ApplicationsWestern Blot
ReactivityMouse, Rat
TargetAKT1
  • SizePrice
Western blot analysis of lysates from Jurkat cells
Product group Antibodies
OriGene
Phospho-AKT1 Rabbit Polyclonal AntibodyTA420069
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetAKT1
  • SizePrice
17-AAG [75747-14-7]
Product group Chemicals
BPS Bioscience
17-AAG [75747-14-7]27027
CAS Number75747-14-7
Estimated Purity≥99%
Molecular Weight585.7 Da
  • SizePrice
Product group Chemicals
BPS Bioscience
PP242 [1092351-67-1]27063
CAS Number1092351-67-1
Estimated Purity≥99%
Molecular Weight308.3 Da
  • SizePrice
Chidamide [743420-02-2]
Product group Chemicals
BPS Bioscience
Chidamide [743420-02-2]27202
CAS Number743420-02-2
Estimated Purity≥98%
Molecular Weight390.4 Da
  • SizePrice
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