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Kinetic Activity: UCHL3 (SBB-DE0023) activity with Ubiquitin-AMC (SBB-PS0043) measured in the presence of 12.5nM and 0.1nM Ubiquitin vinyl sulfone (SBB-PS0032).
Product group Proteins / Signaling Molecules
South Bay Bio
Protein IDP0CG47
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Kinetic Activity: UCHL3 (SBB-DE0023) activity with Ubiquitin-AMC (SBB-PS0043) measured in the presence of 15.0nM and 0.1nM Ubiquitin vinyl methyl ester (SBB-PS0033).
Product group Proteins / Signaling Molecules
South Bay Bio
Protein IDP0CG47
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Kinetic Activity: UCHL3 (SBB-DE0023) activity with Ubiquitin-AMC (SBB-PS0043) measured in the presence of 15.0nM and 0.1nM Ubiquitin propargylamide (SBB-PS0034).
Product group Proteins / Signaling Molecules
South Bay Bio
Protein IDP0CG47
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Product group Proteins / Signaling Molecules
South Bay Bio
Protein IDP0CG47
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Product group Proteins / Signaling Molecules
South Bay Bio
Protein IDP0CG47
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Product group Proteins / Signaling Molecules
South Bay Bio
Protein IDP0CG47
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% Signal to Background of Continuous Real-Time TR-FRET E3 Ligase titration (autoubiquitination): An example using serial dilutions of GST-MDM2 from 200nM to 12.5nM mixed with UBA1, UBE2D3 and trf-Ub mix. Reaction was initiated with addition of Mg-A
Product group Proteins / Signaling Molecules
South Bay Bio
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Product group Proteins / Signaling Molecules
South Bay Bio
Protein IDP0CG47
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Ubiquitinated-p53 Western Blot: From left to right: Control His6-p53 (50ng), ubiquitinated-p53 (50ng and 100ng) and ubiquitinated-p53 digested with USP7 (50ng).
Product group Proteins / Signaling Molecules
South Bay Bio
Protein IDP04637
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% Signal to Background of Continuous Real-Time TR-FRET MDM2 titration (autoubiquitination): Serial dilutions of GST-MDM2 from 200nM to 12.5nM mixed with UBA1, UBE2D3 and trf-Ub mix. Reaction was initiated with addition of Mg-ATP.
Product group Chemicals
South Bay Bio
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% Signal to Background of Continuous Real-Time TR-FRET ITCH titration (autoubiquitination): Serial dilutions of ITCH from 130nM to 8.125nM mixed with UBA1, UBE2D3 and trf-Ub mix. Reaction was initiated with addition of Mg-ATP.
Product group Chemicals
South Bay Bio
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% Signal to Background of Continuous Real-Time TR-FRET Parkin titration (autoubiquitination): Serial dilutions of Parkin W403A from 50nM to 3.125nM and 300nM wt Parkin were mixed with UBA1, UBE2D3 and trf-Ub (pS65) mix. Reactions were initiated wit
Product group Chemicals
South Bay Bio
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