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Product group Proteins / Signaling Molecules
South Bay Bio
Ubiquitin Activating Enzyme E1 [UBA1] (human) (rec.) (untagged)SBB-CE0011
Protein IDP22314
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Thioester Activity Assay: UBE2D3 (UbcH5c) forms a thioester with Ub in an ATP-dependent manner and the bond can be reduced with addition of access DTT. Confirms the activity of UBE2D3.
Product group Proteins / Signaling Molecules
South Bay Bio
UBE2D3 [UbcH5c ] (human) (rec.) (untagged)SBB-CE0019
Protein IDP61077
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Thioester Activity Assay: UBE2L3 (UbcH7) forms a thioester with Ub in an ATP-dependent manner and the bond can be reduced with addition of access DTT. Confirms the activity of UBE2L3.
Product group Proteins / Signaling Molecules
South Bay Bio
UBE2L3 [UbcH7] (human) (rec.) (untagged)SBB-CE0020
Protein IDP68036
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Thioester Activity Assay: UBE2D1 (UbcH5a) forms a thioester with Ub in an ATP-dependent manner and the bond can be reduced with addition of access DTT. Confirms the activity of UBE2D1.
Product group Proteins / Signaling Molecules
South Bay Bio
UBE2D1 [UbcH5a] (human) (rec.) (untagged)SBB-CE0021
Protein IDP51668
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Thioester Activity Assay: UBE2K forms a thioester with Ub in an ATP-dependent manner and the bond can be reduced with addition of excess DTT. The thioester assay also shows di-ubiquitin formation with addition of ATP. This confirms the activity of UBE2K.
Product group Proteins / Signaling Molecules
South Bay Bio
UBE2K [UbcH1, E2-25K] (human) (rec.) (His)SBB-CE0022
Protein IDP61086
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Thioester Activity Assay: UBE2D2 (UbcH5b) forms a thioester with UB in an ATP dependent manner and the bond can be reduced with addition of access DTT. Confirms the activity of UBE2D2.
Product group Proteins / Signaling Molecules
South Bay Bio
UBE2D2 [UbcH5b] (human) (rec.) (untagged)SBB-CE0027
Protein IDP62837
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Ubiquitin Activating Enzyme E1 [UBA1] (human) (rec.) (His)
Product group Proteins / Signaling Molecules
South Bay Bio
Ubiquitin Activating Enzyme E1 [UBA1] (human) (rec.) (His)SBB-CE0058
Protein IDP22314
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Michaelis-Menten Kinetics: Ubiquitin Rhodamine 110 serially diluted from 1.6 to 0.05microM was digested with 30pM UcHL3 over time. The assay was carried out in a reaction buffer of 50mM HEPES pH 7.5, 100mM NaCl, 1mM TCEP, 0.1mg/ml BSA, at 25°C. Initial velocities at each substrate concentration were plotted and fit to the Michaelis-Menten equation. Kinetic parameters were calculated at: Km= 0.69microM, Vmax = 1.29 nMs-1, kcat= 43s-1, kcat/Km= 6.16 x 107 M-1s-1.
Product group Proteins / Signaling Molecules
South Bay Bio
UcH-L3 (human) (rec.) (untagged)SBB-DE0023
Protein IDP15374
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Product group Proteins / Signaling Molecules
South Bay Bio
PLpro (SARS Coronavirus) (rec.) (His)SBB-DE0024
Protein IDP0C6U8
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Michaelis-Menten Kinetics: NEDD8 Rhodamine 110 serially diluted from 1.6 to 0.0125microM was digested with 30pM NEDP1 over time. The assay was carried out in a reaction buffer of 50mM HEPES pH 7.5, 1mM TCEP, 0.1 mg/ml BSA, at 25°C. Initial velocities at each substrate concentration were plotted and fit to the Michaelis-Menten equation. Kinetic parameters were calculated at: Km= 0.087microM, Vmax = 4.44nMs-1, kcat= 148s-1, kcat/Km= 1.70 x 109M-1s-1.
Product group Proteins / Signaling Molecules
South Bay Bio
NEDP1 [SENP8] (human) (rec.) (His)SBB-DE0025
Protein IDQ96LD8
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Product group Proteins / Signaling Molecules
South Bay Bio
SENP1 (catalytic domain) (human) (rec.) (His)SBB-DE0026
Protein IDQ9P0U3
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Product group Proteins / Signaling Molecules
South Bay Bio
20S Immunoproteasome (human) (untagged)SBB-PP0004
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