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Western blot using GeneTex Affinity Purified anti-SFRP1 antibody (GTX24193) shows detection of a band ~37 kDa (arrowhead) corresponding to SFRP1 in lysates from human cultured airway epithelial cells. Lysates were run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:230 dilution of anti-SFRP1 antibody overnight at 4oC. Signal was detected using standard techniques.
Western blot using GeneTex Affinity Purified anti-SFRP1 antibody (GTX24193) shows detection of a band ~37 kDa (arrowhead) corresponding to SFRP1 in lysates from human cultured airway epithelial cells. Lysates were run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:230 dilution of anti-SFRP1 antibody overnight at 4oC. Signal was detected using standard techniques.
Western blot using GeneTex Affinity Purified anti-SFRP1 antibody (GTX24193) shows detection of a band ~37 kDa (arrowhead) corresponding to SFRP1 in lysates from human cultured airway epithelial cells. Lysates were run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:230 dilution of anti-SFRP1 antibody overnight at 4oC. Signal was detected using standard techniques.

SFRP1 antibody

GTX24193
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetSFRP1
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Overview

  • Supplier
    GeneTex
  • Product Name
    SFRP1 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:200-1:2000. ICC/IF: 5 microg/mL. IHC-P: 1:800. ELISA: 1:5000-1:15000. IHC: 1:800. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID6422
  • Target name
    SFRP1
  • Target description
    secreted frizzled related protein 1
  • Target synonyms
    FRP, FRP-1, FRP1, FrzA, SARP2, secreted frizzled-related protein 1, SARP-2, frizzled-related protein, sFRP-1, secreted apoptosis-related protein 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8N474
  • Protein Name
    Secreted frizzled-related protein 1
  • Scientific Description
    SFRP1 is a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. SFRPs act as soluble modulators of Wnt signaling. SFRP1 and SFRP5 may be involved in determining the polarity of photoreceptor cells in the retina. SFRP1 is expressed in several human tissues, with the highest levels in heart.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Cheng LC, Chao YJ, Overman MJ, et al. Increased expression of secreted frizzled related protein 1 (SFRP1) predicts ampullary adenocarcinoma recurrence. Sci Rep. 2020,10(1):13255. doi: 10.1038/s41598-020-69899-8
    Read this paper

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Figure 1. Western blot analysis of SFRP1 using anti-SFRP1 antibody (A01968-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human U2OS whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: rat heart tissue lysates, Lane 5: mouse heart tissue lysates, Lane 6: mouse HEPA1-6 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SFRP1 antigen affinity purified polyclonal antibody (Catalog # A01968-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SFRP1 at approximately 35KD. The expected band size for SFRP1 is at 35KD.
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