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ProductsBack/SPI1 Antibody - middle region (ARP31421_P050)

SPI1 Antibody - middle region (ARP31421_P050)

ARP31421_P050
Aviva Systems Biology
ApplicationsWestern Blot
Product group Antibodies
TargetSPI1
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Overview

  • Supplier
    Aviva Systems Biology
  • Product Name
    SPI1 Antibody - middle region (ARP31421_P050)
  • Delivery Days Customer
    9
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Format
    Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
  • Gene ID6688
  • Target name
    SPI1
  • Target description
    Spi-1 proto-oncogene
  • Target synonyms
    AGM10, OF, PU.1, SFPI1, SPI-1, SPI-A, transcription factor PU.1, 31 kDa transforming protein, hematopoietic transcription factor PU.1, spleen focus forming virus (SFFV) proviral integration oncogene spi1
  • Host
    Rabbit
  • Storage Instruction
    2°C to 8°C,-20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PU.1/SPI1 using anti-PU.1/SPI1 antibody (A01116-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human Raji whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PU.1/SPI1 antigen affinity purified polyclonal antibody (Catalog # A01116-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PU.1/SPI1 at approximately 40 kDa. The expected band size for PU.1/SPI1 is at 40 kDa.
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