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WB analysis of various sample lysates using GTX33530 Suppressor of Fused antibody. Dilution : 1:1000 Loading : 25microg per lane
WB analysis of various sample lysates using GTX33530 Suppressor of Fused antibody. Dilution : 1:1000 Loading : 25microg per lane
WB analysis of various sample lysates using GTX33530 Suppressor of Fused antibody. Dilution : 1:1000 Loading : 25microg per lane

Suppressor of Fused antibody

GTX33530
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetSUFU
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Overview

  • Supplier
    GeneTex
  • Product Name
    Suppressor of Fused antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500 - 1:2000. ICC/IF: 1:50 - 1:200. IHC-P: 1:50 - 1:200. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID51684
  • Target name
    SUFU
  • Target description
    SUFU negative regulator of hedgehog signaling
  • Target synonyms
    JBTS32; negative regulator of hedgehog signaling; PRO1280; SUFUH; SUFUXL; suppressor of fused homolog
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9UMX1
  • Protein Name
    Suppressor of fused homolog
  • Scientific Description
    The Hedgehog signaling pathway plays an important role in early human development. The pathway is a signaling cascade that plays a role in pattern formation and cellular proliferation during development. This gene encodes a negative regulator of the hedgehog signaling pathway. Defects in this gene are a cause of medulloblastoma. Alternative splicing results in multiple transcript variants.[provided by RefSeq, May 2010]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 2. IF analysis of SUFU using anti-SUFU antibody (A02279-1). SUFU was detected in immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2microg/mL rabbit anti-SUFU Antibody (A02279-1) overnight at 4°C. DyLight®488 conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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Lane 1:Mouse intestine lysate Lane 2: Mouse lung lysate Lane 3: Mouse brain probed with Rabbit Anti-SUFU/Suppressor of Fused Polyclonal Antibody, Unconjugated (bs-11209R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.
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