Various tissue extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Synaptophysin antibody [GT2589] (GTX633972) diluted at 1:10000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
![Synaptophysin antibody [GT2589] detects Synaptophysin protein by immunofluorescent analysis. Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Synaptophysin stained by Synaptophysin antibody [GT2589] (GTX633972) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).](https://www.genetex.com/upload/website/prouct_img/normal/GTX633972/GTX633972_42758_20181115_ICC_IF_R_w_23061202_320.webp "Synaptophysin antibody [GT2589] detects Synaptophysin protein by immunofluorescent analysis. Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Synaptophysin stained by Synaptophysin antibody [GT2589] (GTX633972) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).")
![Synaptophysin antibody [GT2589] detects Synaptophysin protein at cell membrane in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. Synaptophysin antibody [GT2589] (GTX633972) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX633972/GTX633972_42758_20170329_IHC-P_M_w_23061202_509.webp "Synaptophysin antibody [GT2589] detects Synaptophysin protein at cell membrane in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. Synaptophysin antibody [GT2589] (GTX633972) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Synaptophysin antibody [GT2589] detects Synaptophysin protein at cell membrane in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. Synaptophysin antibody [GT2589] (GTX633972) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX633972/GTX633972_42758_20170329_IHC-P_R_w_23061202_828.webp "Synaptophysin antibody [GT2589] detects Synaptophysin protein at cell membrane in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. Synaptophysin antibody [GT2589] (GTX633972) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Rat tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Synaptophysin antibody [GT2589] (GTX633972) diluted at 1:10000.](https://www.genetex.com/upload/website/prouct_img/normal/GTX633972/GTX633972_42758_20170223_WB_R_brain_w_23061202_819.webp "Rat tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Synaptophysin antibody [GT2589] (GTX633972) diluted at 1:10000.")
![Human tissue extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Synaptophysin antibody [GT2589] (GTX633972) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX633972/GTX633972_42758_20190823_WB_brain_w_23061202_115.webp "Human tissue extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Synaptophysin antibody [GT2589] (GTX633972) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.")
![Synaptophysin antibody [GT2589] detects Synaptophysin protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse hippocampal CA3. Green: Synaptophysin stained by Synaptophysin antibody [GT2589] (GTX633972) diluted at 1:200. Red: Tau antibody (GTX130462) diluted at 1:250. Blue: Fluoroshield with DAPI (GTX30920). Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX633972/GTX633972_42758_20251114_IHC-P_M_25112100_824.webp "Synaptophysin antibody [GT2589] detects Synaptophysin protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse hippocampal CA3. Green: Synaptophysin stained by Synaptophysin antibody [GT2589] (GTX633972) diluted at 1:200. Red: Tau antibody (GTX130462) diluted at 1:250. Blue: Fluoroshield with DAPI (GTX30920). Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Various tissue extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Synaptophysin antibody [GT2589] (GTX633972) diluted at 1:10000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody. Corresponding RNA expression data are based on NCBI database.](https://www.genetex.com/upload/website/prouct_img/normal/GTX633972/GTX633972_42758_20251128_WB_M_tissue_RPKM_watermark_25112800_985.webp "Various tissue extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Synaptophysin antibody [GT2589] (GTX633972) diluted at 1:10000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody. Corresponding RNA expression data are based on NCBI database.")
Various tissue extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Synaptophysin antibody [GT2589] (GTX633972) diluted at 1:10000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
Synaptophysin antibody [GT2589]
GTX633972
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetSYP
Overview
- SupplierGeneTex
- Product NameSynaptophysin antibody [GT2589]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:5000-1:20000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDGT2589
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID6855
- Target nameSYP
- Target descriptionsynaptophysin
- Target synonymsMRX96, MRXSYP, XLID96, synaptophysin, major synaptic vesicle protein P38
- HostMouse
- IsotypeIgG2b
- Protein IDP08247
- Protein NameSynaptophysin
- Scientific DescriptionSynaptophysin (p38) is an integral membrane protein of small synaptic vesicles in brain and endocrine cells.[supplied by OMIM]
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
Datasheet
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