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TGF alpha Antibody (YA037, Rabbit)

HY-P80911
MedChem Express
TargetTGFA
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    TGF alpha Antibody (YA037, Rabbit)
  • Delivery Days Customer
    10
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID7039
  • Target name
    TGFA
  • Target description
    transforming growth factor alpha
  • Target synonyms
    protransforming growth factor alpha; TFGA; TGF-alpha
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    TGF alpha Antibody (YA037) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to TGF alpha.
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of TGFA using anti-TGFA antibody (M01779-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human A431 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TGFA antigen affinity purified monoclonal antibody (Catalog # M01779-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TGFA at approximately 17 kDa. The expected band size for TGFA is at 17 kDa.
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