Bio-Connect

TNF Alpha Antibody (PE)

LS-C18307
LifeSpan BioSciences
ApplicationsFlow Cytometry
Product group Antibodies
TargetTNF
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    TNF Alpha Antibody (PE)
  • Delivery Days Customer
    23
  • Application Supplier Note
    Suitable for use in Flow Cytometry. Flow Cytometry: Neat. Use 10 ul to label 1E6 cells in 100ul. Membrane permeabilization is required for this application. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.. Flo Suitable for use in Flow Cytometry. Flow Cytometry: Neat. Use 10 ul to label 1E6 cells in 100ul. Membrane permeabilization is required for this application. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
  • Applications
    Flow Cytometry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    RPE
  • Estimated Purity
    ...
  • Gene ID7124
  • Target name
    TNF
  • Target description
    tumor necrosis factor
  • Target synonyms
    APC1 protein; DIF; TNF, macrophage-derived; TNF, monocyte-derived; TNFA; TNF-a; TNF-alpha; TNFSF2; TNLG1F; tumor necrosis factor; tumor necrosis factor ligand 1F; tumor necrosis factor ligand superfamily member 2; tumor necrosis factor-alpha
  • Host
    Rat
  • Isotype
    IgG1
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TNF alpha using anti-TNF alpha antibody (A00002-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: recombinant human TNF alpha protein 1ng. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TNF alpha antigen affinity purified polyclonal antibody (Catalog # A00002-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TNF alpha at approximately 17KD. The expected band size for TNF alpha is at 17KD.
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