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ProductsBack/TNF alpha Rabbit pAb, PE conjugated

TNF alpha Rabbit pAb, PE conjugated

ORB124896
Biorbyt
ApplicationsFlow Cytometry
Product group Antibodies
ReactivityCanine, Equine, Human, Mouse, Porcine
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Overview

  • Supplier
    Biorbyt
  • Product Name
    TNF alpha Rabbit pAb, PE conjugated
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry
  • Applications Supplier
    Flow-Cyt=1ug/Test FC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    RPE
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    TNF alpha Rabbit pAb, PE conjugated
  • Reactivity
    Canine, Equine, Human, Mouse, Porcine
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TNF alpha using anti-TNF alpha antibody (A00002-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: recombinant human TNF alpha protein 1ng. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TNF alpha antigen affinity purified polyclonal antibody (Catalog # A00002-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TNF alpha at approximately 17KD. The expected band size for TNF alpha is at 17KD.
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