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ProductsBack/Wingless Type MMTV Integration Site Family, Member 10B (WNT10B) Polyclonal Antibody

Wingless Type MMTV Integration Site Family, Member 10B (WNT10B) Polyclonal Antibody

CAU21205
Biomatik
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
TargetWNT10B
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Overview

  • Supplier
    Biomatik
  • Product Name
    Wingless Type MMTV Integration Site Family, Member 10B (WNT10B) Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Applications Supplier
    WB; IHC; ICC; IP.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.43 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID7480
  • Target name
    WNT10B
  • Target description
    Wnt family member 10B
  • Target synonyms
    protein Wnt-10b; SHFM6; STHAG8; wingless-type MMTV integration site family, member 10B; WNT-10B protein; WNT-12
  • Host
    Rabbit
  • Protein IDO00744
  • Protein Name
    Protein Wnt-10b
  • Scientific Description
    The Wingless Type MMTV Integration Site Family, Member 10B (WNT10B) Polyclonal Antibody (Species: Human) has been validated for the following applications: WB, IHC.
  • Reactivity Supplier
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of WNT10B using anti-WNT10B antibody (A02574-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human T-47D whole cell lysates, Lane 2: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-WNT10B antigen affinity purified polyclonal antibody (Catalog # A02574-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for WNT10B at approximately 50 kDa. The expected band size for WNT10B is at 43 kDa.
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