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Gel: 8%SDS-PAGE, Lysate: 40 ug, Lane: Human fetal muscle tissue, Primary antibody: CSB-PA164068(WNT10B Antibody) at dilution 1/200 dilution, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 10 seconds
Gel: 8%SDS-PAGE, Lysate: 40 ug, Lane: Human fetal muscle tissue, Primary antibody: CSB-PA164068(WNT10B Antibody) at dilution 1/200 dilution, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 10 seconds
Gel: 8%SDS-PAGE, Lysate: 40 ug, Lane: Human fetal muscle tissue, Primary antibody: CSB-PA164068(WNT10B Antibody) at dilution 1/200 dilution, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 10 seconds

WNT10B Antibody

CSB-PA164068
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse
TargetWNT10B
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Overview

  • Supplier
    Cusabio
  • Product Name
    WNT10B Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID7480
  • Target name
    WNT10B
  • Target description
    Wnt family member 10B
  • Target synonyms
    protein Wnt-10b; SHFM6; STHAG8; wingless-type MMTV integration site family, member 10B; WNT-10B protein; WNT-12
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO00744
  • Protein Name
    Protein Wnt-10b
  • Scientific Description
    The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It may be involved in breast cancer, and its protein signaling is likely a molecular switch that governs adipogenesis. This protein is 96% identical to the mouse Wnt10b protein at the amino acid level.
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of WNT10B using anti-WNT10B antibody (A02574-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human T-47D whole cell lysates, Lane 2: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-WNT10B antigen affinity purified polyclonal antibody (Catalog # A02574-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for WNT10B at approximately 50 kDa. The expected band size for WNT10B is at 43 kDa.
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