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ProductsBack/WNT10B Antibody (aa30-322)

WNT10B Antibody (aa30-322)

LS-C315040
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman
TargetWNT10B
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    WNT10B Antibody (aa30-322)
  • Delivery Days Customer
    23
  • Application Supplier Note
    ELISA 1:100-1:5000; ICC 1:50-500; WB 1:50-400;
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:100 - 1:400) ELISA 1:100-1:5000; ICC 1:50-500; WB 1:50-400;
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.6 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID7480
  • Target name
    WNT10B
  • Target description
    Wnt family member 10B
  • Target synonyms
    SHFM6, STHAG8, WNT-12, protein Wnt-10b, WNT-10B protein, wingless-type MMTV integration site family, member 10B
  • Host
    Rabbit
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of WNT10B using anti-WNT10B antibody (A02574-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human T-47D whole cell lysates, Lane 2: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-WNT10B antigen affinity purified polyclonal antibody (Catalog # A02574-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for WNT10B at approximately 50 kDa. The expected band size for WNT10B is at 43 kDa.
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