More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/WNT10B Polyclonal Antibody
Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (WNT10B) Polyclonal Antibody, Unconjugated (bs-3662R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (WNT10B) Polyclonal Antibody, Unconjugated (bs-3662R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (WNT10B) Polyclonal Antibody, Unconjugated (bs-3662R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

WNT10B Polyclonal Antibody

BS-3662R
Bioss Antibodies
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetWNT10B
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    WNT10B Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID7480
  • Target name
    WNT10B
  • Target description
    Wnt family member 10B
  • Target synonyms
    protein Wnt-10b; SHFM6; STHAG8; wingless-type MMTV integration site family, member 10B; WNT-10B protein; WNT-12
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO00744
  • Protein Name
    Protein Wnt-10b
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Plant MIR156 regulates intestinal growth in mammals by targeting the Wnt/beta-catenin pathway. Li M et al., 2019 Sep 1, Am J Physiol Cell Physiol
    Read more
  • Epidermal Growth Factor Promotes Proliferation and Migration of Follicular Outer Root Sheath Cells via Wnt/beta-Catenin Signaling. Zhang H et al., 2016, Cell Physiol Biochem
    Read more
  • Erbium: YAG laser (2,940 nm) treatment stimulates hair growth through upregulating Wnt 10b and beta-catenin expression in C57BL/6 mice. Ke J et al., 2015, Int J Clin Exp Med
    Read more

Datasheet

Download Datasheet

FAQ

Go to our frequently asked questions

Related products

Product group Antibodies
LifeSpan BioSciences
WNT10B Antibody (aa30-322)LS-C315040
ApplicationsWestern Blot
TargetWNT10B
  • SizePrice
Product group Antibodies
GeneTex
Wnt10b antibody [HL2370]GTX638576
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
TargetWNT10B
  • SizePrice
Product group Antibodies
Biosynth
WNT10B antibody10R-1870
  • SizePrice
Product group Antibodies
MedChem Express
Wnt-10b Antibody (YA3495, Mouse)HY-P83798
TargetWNT10B
  • SizePrice
Product group Antibodies
Biomatik
Wingless Type MMTV Integration Site Family, Member 10B (WNT10B) Polyclonal AntibodyCAU21205
ApplicationsWestern Blot, ImmunoHistoChemistry
TargetWNT10B
  • SizePrice
Gel: 8%SDS-PAGE, Lysate: 40 ug, Lane: Human fetal muscle tissue, Primary antibody: CSB-PA164068(WNT10B Antibody) at dilution 1/200 dilution, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 10 seconds
Product group Antibodies
Cusabio
WNT10B AntibodyCSB-PA164068
ApplicationsWestern Blot, ELISA
ReactivityHuman, Mouse
TargetWNT10B
  • SizePrice
Figure 1. Western blot analysis of WNT10B using anti-WNT10B antibody (A02574-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human T-47D whole cell lysates, Lane 2: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-WNT10B antigen affinity purified polyclonal antibody (Catalog # A02574-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for WNT10B at approximately 50 kDa. The expected band size for WNT10B is at 43 kDa.
Product group Antibodies
Boster Bio
Anti-WNT10B Antibody Picoband(r)A02574-1-CARRIER-FREE
ApplicationsFlow Cytometry, Western Blot
TargetWNT10B
  • SizePrice
Anti-WNT10B Antibody
Product group Antibodies
Antibodies.com
Anti-WNT10B AntibodyA42553
ApplicationsWestern Blot
  • SizePrice
Immunohistochemistry analysis in human cerebral cortex and pancreas tissues using Anti-WNT10B antibody. Corresponding WNT10B RNA-seq data are presented for the same tissues.
Product group Antibodies
Atlas Antibodies
Anti-WNT10B AntibodyHPA055048
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman
TargetWNT10B
  • SizePrice
Product group Antibodies
RayBiotech
Anti-WNT10B Antibody144-64609
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
TargetWNT10B
  • SizePrice