
Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (WNT10B) Polyclonal Antibody, Unconjugated (bs-3662R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
WNT10B Polyclonal Antibody
BS-3662R
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityBovine, Canine, Equine, Human, Mouse, Porcine, Rabbit, Rat
TargetWNT10B
Overview
- SupplierBioss
- Product NameWNT10B Polyclonal Antibody
- Delivery Days Customer16
- ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
- Applications SupplierWB(1:300-5000), ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 ug/ul
- ConjugateUnconjugated
- Gene ID7480
- Target nameWNT10B
- Target descriptionWnt family member 10B
- Target synonymsSHFM6, STHAG8, WNT-12, protein Wnt-10b, WNT-10B protein, wingless-type MMTV integration site family, member 10B
- HostRabbit
- IsotypeIgG
- Protein IDO00744
- Protein NameProtein Wnt-10b
- ReactivityBovine, Canine, Equine, Human, Mouse, Porcine, Rabbit, Rat
- Storage Instruction-20°C
- UNSPSC41116161
References
- Plant MIR156 regulates intestinal growth in mammals by targeting the Wnt/beta-catenin pathway. Li M et al., 2019 Sep 1, Am J Physiol Cell PhysiolRead this paper
- Epidermal Growth Factor Promotes Proliferation and Migration of Follicular Outer Root Sheath Cells via Wnt/beta-Catenin Signaling. Zhang H et al., 2016, Cell Physiol BiochemRead this paper
- Erbium: YAG laser (2,940 nm) treatment stimulates hair growth through upregulating Wnt 10b and beta-catenin expression in C57BL/6 mice. Ke J et al., 2015, Int J Clin Exp MedRead this paper





![Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Wnt10b antibody [HL2370] (GTX638576) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX638576/GTX638576_T-45033_20230512_WB_multiple_B_23051702_755.webp)