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IHC-P analysis of human breast tissue using GTX85815 Wnt8a antibody. Antigen retrieval : Heat-induced antigen retrieval
IHC-P analysis of human breast tissue using GTX85815 Wnt8a antibody. Antigen retrieval : Heat-induced antigen retrieval
IHC-P analysis of human breast tissue using GTX85815 Wnt8a antibody. Antigen retrieval : Heat-induced antigen retrieval

Wnt8a antibody

GTX85815
GeneTex
ApplicationsImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetWNT8A
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Overview

  • Supplier
    GeneTex
  • Product Name
    Wnt8a antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    IHC-P: 10 - 20 microg/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.7 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID7478
  • Target name
    WNT8A
  • Target description
    Wnt family member 8A
  • Target synonyms
    WNT8D, protein Wnt-8a, WNT8d, protein Wnt-8d, wingless-type MMTV integration site family, member 8A
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9H1J5
  • Protein Name
    Protein Wnt-8a
  • Scientific Description
    The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family, and may be implicated in development of early embryos as well as germ cell tumors. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2014]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Wnt8a using anti-Wnt8a antibody (A08392-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human U87 whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates, Lane 3: human SW620 whole cell lysates, Lane 4: human Raji whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat heart tissue lysates, Lane 7: rat C6 whole cell lysates, Lane 8: mouse brain tissue lysates, Lane 9: mouse Neuro-2a whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Wnt8a antigen affinity purified polyclonal antibody (Catalog # A08392-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Wnt8a at approximately 60KD. The expected band size for Wnt8a is at 60KD.
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