![Non-transfected (–) and transfected (+) 293T whole cell extracts were separated by 10% SDS-PAGE, and the membrane was blotted with Wnt8a antibody [HL1648] (GTX637229) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Non-transfected (–) and transfected (+) 293T whole cell extracts were separated by 10% SDS-PAGE, and the membrane was blotted with Wnt8a antibody [HL1648] (GTX637229) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX637229/GTX637229_T-44746_20230609_WB_multiple_B_23062019_750.webp)
Non-transfected (–) and transfected (+) 293T whole cell extracts were separated by 10% SDS-PAGE, and the membrane was blotted with Wnt8a antibody [HL1648] (GTX637229) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Wnt8a antibody [HL1648]
GTX637229
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Zebra Fish
TargetWNT8A
Overview
- SupplierGeneTex
- Product NameWnt8a antibody [HL1648]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:1000-1:10000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDHL1648
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID7478
- Target nameWNT8A
- Target descriptionWnt family member 8A
- Target synonymsWNT8D, protein Wnt-8a, WNT8d, protein Wnt-8d, wingless-type MMTV integration site family, member 8A
- HostRabbit
- IsotypeIgG
- Protein IDQ9H1J5
- Protein NameProtein Wnt-8a
- Scientific DescriptionThe WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family, and may be implicated in development of early embryos as well as germ cell tumors. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2014]
- ReactivityHuman, Zebra Fish
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
![Whole zebrafish extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Wnt8a antibody [HL1648] (GTX637229) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Whole zebrafish extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Wnt8a antibody [HL1648] (GTX637229) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](https://www.genetex.com/upload/website/prouct_img/normal/GTX637229/GTX637229_45131_20230908_WB_Z_23091319_610.webp)
![Wnt8a antibody [HL1648] detects Wnt8a protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded human colon cancer. Wnt8a stained by Wnt8a antibody [HL1648] (GTX637229) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min Wnt8a antibody [HL1648] detects Wnt8a protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded human colon cancer. Wnt8a stained by Wnt8a antibody [HL1648] (GTX637229) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX637229/GTX637229_T-44746_20230925_IHC-P_23100319_207.webp)
![Wnt8a antibody [HL1648] detects Wnt8a protein by immunofluorescent analysis. Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Wnt8a stained by Wnt8a antibody [HL1648] (GTX637229) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920). Scale bar= 10μm. Wnt8a antibody [HL1648] detects Wnt8a protein by immunofluorescent analysis. Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Wnt8a stained by Wnt8a antibody [HL1648] (GTX637229) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920). Scale bar= 10μm.](https://www.genetex.com/upload/website/prouct_img/normal/GTX637229/GTX637229_45131_20231110_ICC_IF_B_23111422_551.webp)







