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Anti-alpha-V integrin [EM01309]

Ab00887-1.1
Absolute Antibody
ApplicationsFlow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetITGAV
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-alpha-V integrin [EM01309]
  • Delivery Days Customer
    7
  • Application Supplier Note
    This pan-alphav-specific antibody has been used to detect alphav integrin chains using ELISA and on Western blots (Goodman et al, 2012), and to specifically stain alphav integrins in FFPE/TMA material (Goodman et al, 2012; Silginer et al, 2016; Roth et al, 2013). Murine antibodies are typically unable to detect FFPE-embedded integrins.
  • Applications
    Flow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    IHC; WB; FC; ELISA
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    EM01309
  • Gene ID3685
  • Target name
    ITGAV
  • Target description
    integrin subunit alpha V
  • Target synonyms
    antigen identified by monoclonal antibody L230; CD51; integrin alpha-V; integrin alphaVbeta3; integrin, alpha V (vitronectin receptor, alpha polypeptide, antigen CD51); MSK8; vitronectin receptor subunit alpha; VNRA; VTNR
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP06756
  • Protein Name
    Integrin alpha-V
  • Scientific Description
    This chimeric mouse antibody was made using the variable domain sequences of the original Rabbit IgG format, for improved compatibility with existing reagents, assays and techniques.
  • Reactivity Supplier
    Human
  • Reactivity Supplier Note
    This antibody was raised by immunising rabbits, isolating B cells from the immunised rabbits and fusing these with a rabbit plasmacytoma cell line (established by Spieker-Polet et al., 1995) to produce stable hybridomas
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Integrin alpha V/ITGAV using anti-Integrin alpha V/ITGAV antibody (A01561-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human A431 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Integrin alpha V/ITGAV antigen affinity purified polyclonal antibody (Catalog # A01561-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Integrin alpha V/ITGAV at approximately 130-140KD. The expected band size for Integrin alpha V/ITGAV is at 130KD.
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