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ProductsBack/Integrin Alpha V Polyclonal Antibody
Human U937 probed with \tIntegrin Alpha V + Beta 3 Antibody, Unconjugated (bs-1310R ) at 0.2ug for 30 minutes followed by incubation with a PE Conjugated secondary (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).
Human U937 probed with \tIntegrin Alpha V + Beta 3 Antibody, Unconjugated (bs-1310R ) at 0.2ug for 30 minutes followed by incubation with a PE Conjugated secondary (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).
Human U937 probed with \tIntegrin Alpha V + Beta 3 Antibody, Unconjugated (bs-1310R ) at 0.2ug for 30 minutes followed by incubation with a PE Conjugated secondary (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).

Integrin Alpha V Polyclonal Antibody

BS-1310R
Bioss
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetITGAV
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Overview

  • Supplier
    Bioss
  • Product Name
    Integrin Alpha V Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), FCM(1:20-100), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID3685
  • Target name
    ITGAV
  • Target description
    integrin subunit alpha V
  • Target synonyms
    CD51, MSK8, VNRA, VTNR, integrin alpha-V, antigen identified by monoclonal antibody L230, integrin alphaVbeta3, integrin, alpha V (vitronectin receptor, alpha polypeptide, antigen CD51), vitronectin receptor subunit alpha
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP06756
  • Protein Name
    Integrin alpha-V
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

References

  • Substrate stiffness directs diverging vascular fates. Wong L et al., 2019 Sep 15, Acta Biomater
    Read this paper
  • Detecting Vulnerable Atherosclerotic Plaques by 68Ga-Labeled Divalent Cystine Knot Peptide. Jiang L et al., 2019 Mar 4, Mol Pharm
    Read this paper
  • Pathways of production and delivery of hepatocyte exosomes. Chen L et al., 2018 Mar, J Cell Commun Signal
    Read this paper
  • Phosphatidylserine-mediated platelet clearance by endothelium decreases platelet aggregates and procoagulant activity in sepsis. Ma R et al., 2017 Jul 10, Sci Rep
    Read this paper
  • Lyn kinase enhanced hepatic fibrosis by modulating the activation of hepatic stellate cells. Li Y et al., 2017, Am J Transl Res
    Read this paper
  • Mechanosignaling activation of TGFbeta maintains intervertebral disc homeostasis. Bian Q et al., 2017, Bone Res
    Read this paper
  • Integrins and heparan sulfate proteoglycans on hepatic stellate cells (HSC) are novel receptors for HSC-derived exosomes. Chen L et al., 2016 Dec, FEBS Lett
    Read this paper
  • Effects of Cationic Microbubble Carrying CD/TK Double Suicide Gene and alphaVbeta3 Integrin Antibody in Human Hepatocellular Carcinoma HepG2 Cells. Li J et al., 2016, PLoS One
    Read this paper
  • Tenascin-C may accelerate cardiac fibrosis by activating macrophages via the integrin alphaVbeta3/nuclear factor-kappaB/interleukin-6 axis. Shimojo N et al., 2015 Oct, Hypertension
    Read this paper

Datasheet

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Figure 1. Western blot analysis of Integrin alpha V/ITGAV using anti-Integrin alpha V/ITGAV antibody (A01561-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human A431 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Integrin alpha V/ITGAV antigen affinity purified polyclonal antibody (Catalog # A01561-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Integrin alpha V/ITGAV at approximately 130-140KD. The expected band size for Integrin alpha V/ITGAV is at 130KD.
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