ProductsBack/Anti-ITGAV Antibody

Anti-ITGAV Antibody

144-02091

ApplicationsWestern Blot, ImmunoHistoChemistry

Product group Antibodies

TargetITGAV

Overview

Supplier
RayBiotech
Product Name
Anti-ITGAV Antibody
Delivery Days Customer
16
Applications
Western Blot, ImmunoHistoChemistry
Certification
Research Use Only
Clonality
Polyclonal
Conjugate
Unconjugated
Gene ID3685
Target name
ITGAV
Target description
integrin subunit alpha V
Target synonyms
antigen identified by monoclonal antibody L230; CD51; integrin alpha-V; integrin alphaVbeta3; integrin, alpha V (vitronectin receptor, alpha polypeptide, antigen CD51); MSK8; vitronectin receptor subunit alpha; VNRA; VTNR
Host
Rabbit
Isotype
IgG
Protein IDP06756
Protein Name
Integrin alpha-V
Scientific Description
ITGAV Polyclonal Antibody
Storage Instruction
-20°C
UNSPSC
12352203

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Figure 1. Western blot analysis of Integrin alpha V/ITGAV using anti-Integrin alpha V/ITGAV antibody (A01561-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human A431 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Integrin alpha V/ITGAV antigen affinity purified polyclonal antibody (Catalog # A01561-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Integrin alpha V/ITGAV at approximately 130-140KD. The expected band size for Integrin alpha V/ITGAV is at 130KD.

Product group Antibodies

Anti-Integrin alpha V/ITGAV Antibody Picoband(r)A01561-2-CARRIER-FREE

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$Human U937 probed with \tIntegrin Alpha V + Beta 3 Antibody, Unconjugated (bs-1310R ) at 0.2ug for 30 minutes followed by incubation with a PE Conjugated secondary (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).$

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References

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