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Western Blot analysis of HeLa cells using Integrin alphaV Polyclonal Antibody
Western Blot analysis of HeLa cells using Integrin alphaV Polyclonal Antibody
Western Blot analysis of HeLa cells using Integrin alphaV Polyclonal Antibody

ITGAV Antibody

CSB-PA003047
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetITGAV
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Overview

  • Supplier
    Cusabio
  • Product Name
    ITGAV Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3685
  • Target name
    ITGAV
  • Target description
    integrin subunit alpha V
  • Target synonyms
    CD51, MSK8, VNRA, VTNR, integrin alpha-V, antigen identified by monoclonal antibody L230, integrin alphaVbeta3, integrin, alpha V (vitronectin receptor, alpha polypeptide, antigen CD51), vitronectin receptor subunit alpha
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP06756
  • Protein Name
    Integrin alpha-V
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Integrin alpha V/ITGAV using anti-Integrin alpha V/ITGAV antibody (A01561-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human A431 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Integrin alpha V/ITGAV antigen affinity purified polyclonal antibody (Catalog # A01561-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Integrin alpha V/ITGAV at approximately 130-140KD. The expected band size for Integrin alpha V/ITGAV is at 130KD.
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