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Anti-IL-2 [UFKA-20]

Ab02947-10.3
Absolute Antibody
ApplicationsFlow Cytometry, ELISA
Product group Antibodies
ReactivityHuman
TargetIL2
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-IL-2 [UFKA-20]
  • Delivery Days Customer
    7
  • Application Supplier Note
    This antibody binds to Interleukin-2, a protein produced by T-cells in response to antigenic or mitogenic stimulation. IL-2/UFKA-20 complex shows an improved selectivity towards regulatory T cells compared to IL-2 alone. IL-2/UFKA-20 can recognize the receptor IL of regulatory T cells on the base of the presence of CD25, therefore called CD25 bias. IL-2/UFKA-20 complex is able to efficiently stimulate CD25+ Treg cells in mice and rhesus macaques in vivo as well as in humans ex vivo.
  • Applications
    Flow Cytometry, ELISA
  • Applications Supplier
    ELISA; FC
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    UFKA-20
  • Gene ID3558
  • Target name
    IL2
  • Target description
    interleukin 2
  • Target synonyms
    IL-2, TCGF, lymphokine, interleukin-2, T cell growth factor, aldesleukin, involved in regulation of T-cell clonal expansion
  • Host
    Human
  • Isotype
    IgG1
  • Protein IDP60568
  • Protein Name
    Interleukin-2
  • Reactivity
    Human
  • Reactivity Supplier
    Human
  • Reactivity Supplier Note
    The original antibody was generated immunizing Balb/c mice with human IL-2.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of IL-2 using anti-IL-2 antibody (A00387-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: HELA whole Cell lysates, Lane 2: K562 whole Cell lysates, Lane 3: RAJI whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL-2 antigen affinity purified polyclonal antibody (Catalog # A00387-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL-2 at approximately 22KD. The expected band size for IL-2 is at 18KD.
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