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Immunohistochemical staining of human colon shows strong nuclear and cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human colon shows strong nuclear and cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human colon shows strong nuclear and cytoplasmic positivity in glandular cells.

Anti-PAF1 Antibody

HPA041875
Atlas Antibodies
ApplicationsImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
ReactivityHuman
TargetPAF1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-PAF1
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID54623
  • Target name
    PAF1
  • Target description
    PAF1 homolog, Paf1/RNA polymerase II complex component
  • Target synonyms
    F23149_1; Paf1, RNA polymerase II associated factor, homolog; pancreatic differentiation protein 2; PD2; RNA polymerase II-associated factor 1 homolog
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8N7H5
  • Protein Name
    RNA polymerase II-associated factor 1 homolog
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Western Blot Positive WB detected in: HepG2 whole cell lysate, Hela whole cell lysate, K562 whole cell lysate, Mouse brain tissue All lanes: PAF1 antibody at 3.2microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 60, 56 kDa Observed band size: 60, 48 kDa
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Figure 1. Western blot analysis of PAF1 using anti-PAF1 antibody (A01640-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human Caco-2 whole cell lysates, Lane 4: human SW620 whole cell lysates, Lane 5: rat heart tissue lysates, Lane 6: mouse heart tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PAF1 antigen affinity purified polyclonal antibody (Catalog # A01640-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PAF1 at approximately 80KD. The expected band size for PAF1 is at 60KD.
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