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PAF1 Polyclonal Antibody

CAC14324
Biomatik
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetPAF1
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Overview

  • Supplier
    Biomatik
  • Product Name
    PAF1 Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID54623
  • Target name
    PAF1
  • Target description
    PAF1 homolog, Paf1/RNA polymerase II complex component
  • Target synonyms
    F23149_1; Paf1, RNA polymerase II associated factor, homolog; pancreatic differentiation protein 2; PD2; RNA polymerase II-associated factor 1 homolog
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8N7H5
  • Protein Name
    RNA polymerase II-associated factor 1 homolog
  • Scientific Description
    The PAF1 Polyclonal Antibody (Species: Human) has been validated for the following applications: ELISA, WB, IHC.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PAF1 using anti-PAF1 antibody (A01640-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human Caco-2 whole cell lysates, Lane 4: human SW620 whole cell lysates, Lane 5: rat heart tissue lysates, Lane 6: mouse heart tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PAF1 antigen affinity purified polyclonal antibody (Catalog # A01640-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PAF1 at approximately 80KD. The expected band size for PAF1 is at 60KD.
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