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PAF1 Antibody (YA2505, Rabbit)

HY-P82760
MedChem Express
TargetPAF1
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    PAF1 Antibody (YA2505, Rabbit)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID54623
  • Target name
    PAF1
  • Target description
    PAF1 component of Paf1/RNA polymerase II complex
  • Target synonyms
    F23149_1, PD2, RNA polymerase II-associated factor 1 homolog, PAF1 homolog, Paf1/RNA polymerase II complex component, Paf1, RNA polymerase II associated factor, homolog, pancreatic differentiation protein 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    PAF1 Antibody (YA2505) is a non-conjugated IgG antibody, targeting PAF1, with a predicted molecular weight of 60 kDa (observed band size: 80 kDa). PAF1 Antibody (YA2505) can be used for WB experiment in human, mouse, rat background.
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of PAF1 using anti-PAF1 antibody (A01640-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human Caco-2 whole cell lysates, Lane 4: human SW620 whole cell lysates, Lane 5: rat heart tissue lysates, Lane 6: mouse heart tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PAF1 antigen affinity purified polyclonal antibody (Catalog # A01640-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PAF1 at approximately 80KD. The expected band size for PAF1 is at 60KD.
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