Anti-PD-L1 [332M7]

Absolute Antibody

Anti-PD-L1 [332M7]

9

This antibody strongly binds human and cynomolgus monkey PD-L1 and shows weak binding for mouse PD-L1. PD-L1 is highly expressed in many human tumors. . PD-L1 exerts an immune suppressive signal through binding to PD-1 and B7.1, and tumor expression of PD

The binding characterization of this antibody towards human, mouse and cynomolgus monkey PD-L1 was done using flow cytometry. This antibody was shown to block the binding of hPD-1-Fc protein to hPD-L1 expressed on the surface of the HEK-293T cells at a concentration of 10 microg/ml. The mouse version of this antibody was reported to inhibit patient derived melanoma tumor in tumor-bearing mice. There was also found an increase in the gene expression of CD8 and IFN-gamma in tumor samples from mice treated with mouse version of this antibody (US20180346571).

Flow Cytometry, Neutralisation/Blocking

Research Use Only

Monoclonal

332M7

CD274

CD274 molecule

B7 homolog 1; B7-H; B7H1; CD274 antigen; hPD-L1; PDCD1 ligand 1; PDCD1L1; PDCD1LG1; PDL1; PD-L1; programmed cell death 1 ligand 1

Mouse

IgG2b

Programmed cell death 1 ligand 1

This chimeric mouse antibody was made using the variable domain sequences of the original Human IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Human, Monkey, Mouse

-20°C,2°C to 8°C

12352203