Anti-PD-L1 [AB1]

Absolute Antibody

Anti-PD-L1 [AB1]

9

This antibody binds human PD-L1.

The scFv version of this antibody binds human B7-H1 with an affinity of 2.88 nM. The ability of this antibody to block binding of PD-1-Fc protein or B7.1-Fc protein to B7-H1-Fc was evaluated using ELISA. This antibody could effectively block B7-H1/PD-1 binding and B7-H1/B7.1 binding with an IC50 value of 1.37microg/ml and 0.521.37microg/ml respectively. The ability of the AB1 to bind to human B7-H1, mouse B7-H1 was determined by flow cytometric analysis. This antibody did not exhibit any binding to 293F cells transfected with mouse B7-H1, human PD-1 or human ICOS. This antibody was also shown to IFN-gamma release in a PBMC asssay. It was also reported that this antibody can inhibit A375 human melanoma tumor growth in mouse model (WO2015195163).

Flow Cytometry, ELISA

Research Use Only

Monoclonal

AB1

CD274

CD274 molecule

B7 homolog 1; B7-H; B7H1; CD274 antigen; hPD-L1; PDCD1 ligand 1; PDCD1L1; PDCD1LG1; PDL1; PD-L1; programmed cell death 1 ligand 1

Mouse

IgG2b

Programmed cell death 1 ligand 1

This full-length, reformatted mouse antibody was made using the variable domain sequences of the original Mouse scFv format, for improved compatibility with existing reagents, assays and techniques.

Human

-20°C,2°C to 8°C

12352203