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Anti-WNT10B Antibody

144-64609
RayBiotech
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
Product group Antibodies
TargetWNT10B
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-WNT10B Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Gene ID7480
  • Target name
    WNT10B
  • Target description
    Wnt family member 10B
  • Target synonyms
    SHFM6, STHAG8, WNT-12, protein Wnt-10b, WNT-10B protein, wingless-type MMTV integration site family, member 10B
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO00744
  • Protein Name
    Protein Wnt-10b
  • Scientific Description
    WNT10B pAb
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of WNT10B using anti-WNT10B antibody (A02574-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human T-47D whole cell lysates, Lane 2: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-WNT10B antigen affinity purified polyclonal antibody (Catalog # A02574-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for WNT10B at approximately 50 kDa. The expected band size for WNT10B is at 43 kDa.
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