![Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with COX4 antibody [GT6310] (GTX628886) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody. Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with COX4 antibody [GT6310] (GTX628886) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX628886/GTX628886_44503_20211112_WB_22110201_302.webp)
Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with COX4 antibody [GT6310] (GTX628886) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
COX4 antibody [GT6310]
GTX628886
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Rat
TargetCOX4I1
Overview
- SupplierGeneTex
- Product NameCOX4 antibody [GT6310]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDGT6310
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID1327
- Target nameCOX4I1
- Target descriptioncytochrome c oxidase subunit 4I1
- Target synonymsCOX IV-1, COX4, COX4-1, COXIV, COXIV-1, MC4DN16, cytochrome c oxidase subunit 4 isoform 1, mitochondrial, cytochrome c oxidase polypeptide IV, cytochrome c oxidase subunit IV
- HostMouse
- IsotypeIgG2b
- Protein IDP13073
- Protein NameCytochrome c oxidase subunit 4 isoform 1, mitochondrial
- Scientific DescriptionCytochrome c oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. The complex consists of 13 mitochondrial- and nuclear-encoded subunits. The mitochondrially-encoded subunits perform the electron transfer and proton pumping activities. The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex. This gene encodes the nuclear-encoded subunit IV isoform 1 of the human mitochondrial respiratory chain enzyme. It is located at the 3 of the NOC4 (neighbor of COX4) gene in a head-to-head orientation, and shares a promoter with it. [provided by RefSeq]
- ReactivityHuman, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Naeem S, Qi Y, Tian Y, et al. NIX compensates lost role of parkin in cd-induced mitophagy in HeLa cells through phosphorylation. Toxicol Lett. 2020,326:1-10. doi: 10.1016/j.toxlet.2020.03.001Read this paper
- Choi BW, Jeong YJ, Park SH, et al. Reverse Warburg Effect-Related Mitochondrial Activity and (18)F-FDG Uptake in Invasive Ductal Carcinoma. Nucl Med Mol Imaging. 2019,53(6):396-405. doi: 10.1007/s13139-019-00613-xRead this paper
- Gu X, Qi Y, Feng Z, et al. Lead (Pb) induced ATM-dependent mitophagy via PINK1/Parkin pathway. Toxicol Lett. 2018,291:92-100. doi: 10.1016/j.toxlet.2018.04.012Read this paper
- Jeong SA, Kim IY, Lee AR, et al. Ca2+ influx-mediated dilation of the endoplasmic reticulum and c-FLIPL downregulation trigger CDDO-Me-induced apoptosis in breast cancer cells. Oncotarget. 2015,6(25):21173-92.Read this paper
![COX4 antibody [GT6310] detects COX4 protein at mitochondria by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. COX4 stained by COX4 antibody [GT6310] (GTX628886) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min COX4 antibody [GT6310] detects COX4 protein at mitochondria by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. COX4 stained by COX4 antibody [GT6310] (GTX628886) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX628886/GTX628886_44503_20230303_IHC-P_23032819_402.webp)

![COX4 antibody [GT6310] detects COX4I1 protein at cytosol on U87 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded U87 xenograft. COX4 antibody [GT6310] (GTX628886) dilution: 1:200.
Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min COX4 antibody [GT6310] detects COX4I1 protein at cytosol on U87 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded U87 xenograft. COX4 antibody [GT6310] (GTX628886) dilution: 1:200.
Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min](https://www.genetex.com/upload/website/prouct_img/normal/GTX628886/GTX628886_41239_IHC_w_23061202_134.webp)
![COX4 antibody [GT6310] detects COX4I1 protein at cytosol on human hepatoma by immunohistochemical analysis. Sample: Paraffin-embedded hepatoma. COX4 antibody [GT6310] (GTX628886) dilution: 1:200.
Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min COX4 antibody [GT6310] detects COX4I1 protein at cytosol on human hepatoma by immunohistochemical analysis. Sample: Paraffin-embedded hepatoma. COX4 antibody [GT6310] (GTX628886) dilution: 1:200.
Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min](https://www.genetex.com/upload/website/prouct_img/normal/GTX628886/GTX628886_41239_IHC_2_w_23061202_403.webp)
![COX4 antibody [GT6310] detects COX4I1 protein by Western blot analysis. A. 50 μg rat muscle lysate/extract 12 % SDS-PAGE COX4 antibody [GT6310] (GTX628886) dilution: 1:1000 COX4 antibody [GT6310] detects COX4I1 protein by Western blot analysis. A. 50 μg rat muscle lysate/extract 12 % SDS-PAGE COX4 antibody [GT6310] (GTX628886) dilution: 1:1000](https://www.genetex.com/upload/website/prouct_img/normal/GTX628886/GTX628886_41239_WB_R_muscle_w_23061202_539.webp)
![Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with COX4 antibody [GT6310] (GTX628886) diluted at 1:1500. Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with COX4 antibody [GT6310] (GTX628886) diluted at 1:1500.](https://www.genetex.com/upload/website/prouct_img/normal/GTX628886/GTX628886_41239_20160721_WB_shRNA_watermark_w_23061202_858.webp)






